Xenopus is the leading vertebrate model for the analysis of gene function in development. The combination of lineage analysis, gene-knockout strategies, experimental manipulation of the embryo, and genomic/bioinformatic techniques, makes it ideal for studies on the molecular control of embryo patterning, morphogenesis and organogenesis. Moreover, recent advances in Xenopus genomics offer new opportunities to integrate computational strategies with experimental approaches. The course combines intensive laboratory training with daily lectures from recognized experts in the field. Students will learn both emerging technologies and classical techniques to study gene function in Xenopus development. An important element will be the informal interaction between students and course faculty.
Technologies to be covered will include: oocyte and embryo culture, lineage analysis and experimental manipulation of embryos, time lapse imaging of morphogenesis, gain and loss of function analysis using mRNAs and antisense oligonucleotides, whole mount in situ hybridization, immunocytochemistry, genomics and bioinformatics, chromatin immunoprecipitation, preparation of transgenic embryos, and use of Xenopus tropicalis for genetic analyses. The Cell and Developmental Biology of Xenopus course is designed for those new to the Xenopus field, as well as for those wanting a refresher course in the emerging technologies. The course is open to investigators from all countries.
Lecturers for the 2012 course included: Heithem El-Hodiri, Michael Gilchrist, Robert Grainger, Raymond Keller, Mustafa Khokha, Peter Klein, Jonathan Slack, John Wallingford, Lyle Zimmerman
This course is supported with funds provided by the National Institute of Child Health and Human Development.