Cell and Developmental Biology of Xenopus
Paul Krieg, University of Arizona
Sally A. Moody, The George Washington University Medical Center
April 8-18, 2000
The frog Xenopus is an important vertebrate model for studies of maternal factors, regulation and molecular mechanisms of tissue inductions and regulation of cell fate decisions. In addition, Xenopus oocytes and embryos provide a powerful system in which to conduct a number of cell biological and gene regulation assays. This course will provide extensive laboratory exposure to the biology, manipulation and use of oocytes and embryos of Xenopus. The course consists of intensive laboratory sessions, supplemented by daily lectures and demonstrations from experts in cellular, experimental and molecular development. Areas to be covered include: (i) care of adults; ii) oocyte isolation and embryo production; (iii) stages of embryonic development and anatomy; (iv) whole mount in situ hybridization and immunocytochemistry; (v) microinjection of eggs and oocytes with lineage tracers, DNA constructs, mRNA and antisense oligonucleotides; (vi) micromanipulation of embryos, including explant and transplantation assays; (vii) preparation of transgenic embryos; and (viii) use of Xenopus tropicalis for genetic analyses. This course is suited both for investigators who have had no experience with Xenopus, as well as those who have worked with Xenopus and wish to learn new and cutting edge techniques. All applicants should have current training in molecular biology and some knowledge of developmental biology
Y2K guest lecturers and assistants included: Enrique Amaya, Cambridge University; Ondine Cleaver, Harvard Medical School; Robert Grainger, University of Virginia; Sir John Gurdon, Wellcome/CRC Institute, Cambridge; Janet Heasman, University of Minnesota; Raymond Keller, University of Virginia; Kristy Kenyon, The George Washington University; Mary Lou King, University of Miami; Petra Pandur, University of Ulm; Aaron Zorn, Wellcome/CRC Institute, Cambridge.
Application Deadline is January 15, 2000.
Visit our web site for details on how to apply and for prices and possible scholarship support: http://www.cshl.org/meetings/2000courses.htm
The Cold Spring Harbor Laboratory Meetings Office, 1 Bungtown Road, Cold Spring Harbor, NY 11724-2213. tel 516-367-8343, fax 516-367-8845. meetings@cshl.org