Xenopus Axin, Xaxin. Midbrain
Axin, nucleic acid
Xaxin is expressed maternally and throughout early development but is highly enriched in the
anterior midbrain at the tailbud stage. These whole mounts were done by Matt Deardorff; the probe is Xenopus axin in pCS2 cloned by Chester Hedgepeth. Images and legends submitted by Peter Klein.
- a. Xaxin, lateral view, tailbud: High expression in the anterior midbrain.
Lower level of expression throughout tadpole with longer chromophore
reaction.
- b. Xaxin, dorsal view, tailbud: High expression is in the anterior
midbrain. Lower level of expression throughout tadpole with longer
chromophore reaction.
- c. Xaxin + Xwnt2B, lateral view: (Xaxin is caudal to Xwnt2B; Xwnt2B per Y.
Landesman and S. Sokol)
- d. Xaxin + Xwnt2B, dorsal view: posterior crescent is Xaxin expression in
the anterior midbrain and anterior crescent is Xwnt2B in the posterior
forebrain (Color difference for two probes did not reproduce well).
- e. Xaxin+ engrailed, Lateral view: Xaxin is the rostral darker staining
(filled arrow) and engrailed is the caudal lighter staining (open arrow;
engrailed from A. Hemmati-Brivanlou and R. Harland).
- Available from Peter Klein
- Sense probes are made by NotI digest and SP6 polymerase
- Antisense probes are made by PstI digest (followed by fill-in) and T7 polymerase
- GenBank #AF097313
- Reference: Hedgepeth, C.M., Deardorff, M.A., and P.S. Klein (1998) Xenopus axin interacts with glycogen synthase kinase-3 beta and is expressed in the anterior midbrain. Mechanisms of Development 80: 147-151.
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