Xenopus cpl-1; nervous system, neural

Xenopus choroid plexus lipocalin I, Xl cpl-1, nucleic acid

Pictures from Anne Knecht and Richard Harland, text from Peter Good and Anne Knecht.

Developmental Northern Blot for cpl-1.
The cDNA corresponding to the cpl-1 gene (13-6) was isolated in a screen for markers of the embryonic nervous system. The gene is activated shortly after gastrulation (Northern Blot). The predicted protein encoded by the 13-6 cDNA is a member of the lipocalin gene family, whose members function as ligand carriers. Xenopus cpl-1 is expressed in the adult choroid plexus and is homologous to a gene identified in Bufo marinus that is the predominant protein secreted by the choroid plexus.
The high resolution files are approx. 0.2 MB compressed
Stage 13 Stage 23
Forebrain X-section
Approx. 0.3 MB compressed.
By whole mount in situ hybridization, cpl-1 is expressed at stage 13 in a stripe at the anterior edge of the neural plate (stage 13, dorsal view). This staining persists as the neural folds close, with expression ending up mainly in the dorsal forebrain, and trailing off posteriorly down the dorsal midline of the brain (stage 23 lateral view; blue stain indicates cpl-1 expression while brown stain marks the cement gland (XAG-1)). The level of expression peaks around stage 23 and afterward declines with no change in pattern. Sections reveal that cpl-1 is strictly brain specific, with no expression in spinal cord or non-neural ectoderm (Forebrain X-section). Thus cpl-1 marks only dorsal brain, and mainly dorsal forebrain.

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  • Reference: Knecht, A.K., Good, P.J., Dawid, I.B., and Harland, R.M. (1995). Dorsal-Ventral Patterning and Differentiation of Noggin-Induced NeuralTissue in the Absence of Mesoderm Development 121, 1927-1936.
    Richter, K., H. Grunz, and I.B. Dawid. 1988. Gene expression in the embryonic nervous system of Xenopus laevis. Proc. Natl. Acad. Sci. 85:8086-8090.
  • Plasmid name; p13-6 (download restriction map)
  • cpl-1 cDNA (p13-6) in pBluescriptKS
  • download sequnce
  • Genbank accession number; X84414.
  • For in situ probe cut with BamHI and transcribe with T3
  • Available from Peter Good

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