int-2; nervous system
int-2 (fgf-3), nucleic acid
by Harry Isaacs. Developmental Biology Program, Dept Biology and Biochemistry, University of Bath, Bath BA2 7AY United Kingdom.
int-2 (fgf-3) expression is first detected in a ring around the blastopore
early during gastrulation.
Figure 1 Vegetal view, stage 10.5. (dorsal to the top) At
this stage there is low level expression of int-2 around the closing
blastopore. During later gastrula stages int-2 is expressed at much higher
levels in the lateral and ventral blastopore lip. This pattern is complementary to the
expression of eFGF which is expressed at higher levels in the
dorsal lip region during these stages.
Figure 2 Dorsal view, stage 13. By the end of gastrulation int-2 is highly expressed in the posterior of the embryo and in a stripe in the neural plate at the level of the hindbrain. Note that unlike eFGF, int-2 expression is not seen in the dorsal midline.
Figure 3. Dorsal view, stage 20. int-2 continues to be expressed as a stripe in the hindbrain region. Also (not visible in this image) int-2 is expressed in the developing tailbud.
- Probe generation. In situs hybridised with 297 base probe from Tannahill et al., 1992.
- Linearise with BamHI, transcribe with T3 polymerase. Do not RNase treat embryos.
- Available from Harry Isaacs, Developmental Biology Program, Dept Biology and Biochemistry, University of Bath, Bath BA2 7AY United Kingdom
- Reference; Tannahill, D., Isaacs, H.V., Close, M.J., Peters, G. and Slack, J.M.W. (1992) Developmental expression of the Xenopus int-2 (FGF-3) gene: activation by mesodermal and neural induction. Development 115, 695-702.
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Figure 3. Dorsal view, stage 20. int-2 continues to be expressed as a stripe in the hindbrain region. Also (not visible in this image) int-2 is expressed in the developing tailbud.