Monoclonal Tor70; lateral line, notochord

Monoclonal Tor70, lateral line

Recent characterization by Peg Bolce and Richard Harland (1992). Tor70 had previously been found to stain zebrafish notochord (P.D. Kushner and B. Mendelson, unpublished information). In Xenopus the antibody is specific to differentiating notochord from the late neurula stage to about stage 28. All the vacuolating notochord cells which are detectable by Nomarski microscopy of cleared specimens stain with the antibody. The notochord staining declines, as staining appears in the otic vesicle, some cranial ganglia and a few proctodeal and stomodeal cells. At stage 40, staining is absent from the notochord, but prominent in lateral line, proctodeum, stomodeum and nasal pits. Also works on urodeles (Vize unpubl). Goodrich's sketch of fish lateral line.
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Antibody 2G9 also stains the lateral line

Xenopus

A. B. C.
A. Stage 38 lateral view of anterior end. B. Closer shot of the head stain in the infraorbital lateral line. The more posterior row of stained cells is probably the mandibular line. Nasal pit is also obvious, even though out of focus. (stage 39). C. The nasal pits, dorsal view (stage 41)

Axolotl

A. B. C.
A. Stage 38 nasal pit, dorsal view . B. Even closer (dorsal view). C. The otic stain (dorsal view) at stage 38-39. This is probably just background (common in the ear) but it shows otic structure quite nicely.
all of these images are 100% digital, and were captured directly from the microscope by a NeXTDimension computer system
Original Reference; Kushner, P.D., J.Neurochemistry 43: 775-786
Recent Reference; Bolce et al., Development 115, 681-688. Other lateral line refs; Northcutt, R.G., Brandle, K. and Fritzsch, B. (1995) Electroreceptors and mechanosensory lateral line organs arise from single placodes in axolotls. Devl.Biol. 168:358-373

Search PubMed for papers by Richard Harland
or for other papers in which tor70 appears in the abstract

For additional information on this antibody please visit the full Tor70 page

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