tubulin; ciliated cells, mitotic figures
tubulin, monoclonal
A commercial antibody form Amersham gives excellent results, catalog#17513, as does the antibody from the DSHB. In blastula and gastrula excellent for detecting mitotic indices. Tubulin is everywhere of course but the antibody is very good for staining microtubule rich structures in latter development, two examples shown below are ciliated epidermal cells, and pronephric nephrostomes. Information and pictures from Peter Vize.
A. 
A. blastula, lateral view.
B. 
, C. 
B. Gastrula, looking down on the animal pole. C. Close up of similar sample.
D. 
E. 
D. stage 22 tailbud, dorsal view. Patches of ciliated epidermis are visible. E. Lateral view. By stage 23/24 the entire epidermis is covered with evenly spaced ciliated cells. This is actually an axolotl embryo, but equivalent Xenopus stages look the same.
F. 
F. It's hard to see in this photo (better ones will be on-line soon), but part of the pronephros (just below the indicator) stains. This is probably the ciliated nephrostomal funnels, which sweep fluids from the coelom into the pronephric tubules.
DSHB
Peter
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, C. 
E. 
