Marker index. Please send more images/plasmids/libraries etc!

By tissue or staining pattern

• apoptosis; TUNEL staining patterns
• blood, blood islands; globin, GATA-2, GATA-4, benzidine histochemical stain
• blood vessels; ets-1/ets-2, Xl-fli, (more soon!)
• branchial arches; see neural crest
• brain; axin, Xwnt-4, nrp-1, N-myc, Xen1
• cement gland; XA, XAG, XCG
• Cell cortex; beta catenin, plaktoglobin
• cranial ganglia (any); Tor70, nrp-1,
• cyclins;cyclin D1
• dorsal lip; chordin, Xnr3, goosecoid, noggin
• early mesoderm; Mix, xbra, BMP2, BMP4, Xwnt-8, chordin, Xnr3, eFGF, noggin, XVent1 and XVent2
• endothelial cells, blood vessels; ets-1/ets-2, Xl-fli
• epidermal; epi1
• eye; cyclinD1, N-myc, Xen1
• glomus; xWT1 (Wilms'), glomus anatomy page
• growth factors; BMP2, BMP4, eFGF, int-2 (fgf-3)
• germ cells and/or germ plasm; Xpat
• germinal vesicle; nuclear lamin
• gut;
• hatching gland; XA, XAG
• heart; cardiac actin, cardiac troponin, XMHC alpha, GATA-4, XNKX-2.5, XTin1, Xl-fli (endocardium!)
• hindbrain (restricted), int-2
• lateral line; Tor70, 2G9
• lateral plate; XTwist, XSlug
• liver; Xhex
• mesonephros; xWT1 (Wilms')
• midbrain; axin
• muscle;12/101, 5A3, XMyf-5, XMyoD
• nasal pits; Tor70, BMP2
• neural (CNS); axin, HNK-1, HoxB9 (=XlHbox6), HoxD1, nrp-1, N-CAM, 2G9, Xen-1, NF-M, class II beta tubulin, tanabin, synaptobrevin II, cpl-1, etr-1, int-2, Xen1
• neural (PNS); HNK-1, shaker-1, islet-1
• neural crest; ets-1, ets-2, gremlin, Slug, XTwist, xAP2, PCNA (not specific but helps visualize), N-myc, Mad
• notochord; Xnot, Tor70, MZ15, shh
• nucleus; nuclear lamin
• ORGANIZER; noggin, goosecoid, Xlim-1, Xnot, XLPOU 2, Xotx2, chordin, nodal related Xnr3 , noggin
• otic vesicle; 3G8; Tor70, BMP4, Xwnt-3A and Xwnt-4 , XPax-2, XPax-8
• pancreas; xlhbox8
• pcd; TUNEL staining of pcd
• pituitary;
• primordial germ cells; Xpat
• pronephros;3G8 (11.8), XSMP-30, xWT1 (glomus only), Xwnt-4, HNF1 beta, XDCoH, N-myc, Xlim-1, XPax-2, XPax-8
• pronephric duct; 4A6 , ets-2, gremlin, XPax-2
• rectal diverticulum; XPax-2
• retina; membrane skeletal protein 4.1 (pab)
• somitomeres; X-Delta-2
• spinal chord (not brain); HoxB9 (=XlHbox6)
• spleen;
• ventral mesoderm: XVent1 and XVent2

By Marker

If the marker has a page in this catalog you can jump to it by clicking on the coloured link.

Monoclonal antibodies

• 2G9 (Jones); pan neural.
• nuclei/germinal vesicle; anti-nuclear lamin
• 3G8 (Vize et al., 1995); pronephric tubules and otic vesicle. stg 31/32 onwards.
• 4A6; (Vize et al., 1995); pronephric duct. stg 38/39 onwards
• 5A3 (Jones); somite and heart in tailbud. Other muscles from stage 38/39
• 12/101 (Kintner and Brockes 1984); somite (not in heart). stg 17 onwards. Other muscles from stage 38/39(DSHB)
• HNK-1
• PCNA, proliferating cell nuclear antigen. Commercial from DAKO. Stains "dividing" cells. Actually stains lots of cells, many of which have no intention of dividing. Useful for visualizing migrating crest and some other cell types.
• tor70 (Kushner, 1984); notochord to about stage 28. From stage 40, lateral line, some cranial ganglia, proctodeum, stomodeum and nasal pits.
• tubulin; mitotic spindles, ciliated cells (e.g. nephrostomes, ciliated epidermis). (commercial from Amersham, cat#17513)
• Xen1 (Ruiz i Altaba); CNS

Polyclonal antibodies

• membrane skeletal protein 4.1 (rabbit); detects post-mitotic cells of retina
• neurofilament (rabbit); CNS, also detects ciliated epidermis.
• N-myc; brain, eye, crest, weaker somite and pronephros.

Nucleic acids

• axin; throughout development, very high in the midbrain in tailbud stages
• XA; cement gland and hatching gland
• XAG; cement gland and hatching gland
• BMP; somite, pineal gland, base of cement gland, part of heart, fin, others.
• BMP4; early in ventro-lateral mesoderm, later in various parts of head including otic, anterior cresent of the eye, base of the cement gland, others.
• xbra (brachyury); mesoderm from MBT to stage 10, from stage 10 mesoderm blastoporal mesoderm and presumptive notochord.
• cardiac actin; somite and heart. stg 13 onwards.
• cardiac troponin I; heart stage 28 to adult.
• beta catenin; cell cortex
• XCG; cement gland
• chordin; dorsal lip, dorsal mesoderm, neural plate
• cpl-1, dorsal brain, mostly dorsal forebrain
• cyclin D1, anterior CNS, eye, crest.
• X-Delta-2; prospective somite boundries, somitomeres
• eFGF:
• etr-1, CNS, including eyes, epiphysis, nasal pits and cranial ganglia and nerves.
• ets-1; neural crest, cranial ganlia, endothelial cells, later in aortic arches and intersomitic vessels
• ets-2, pronephric tubules and pronephric duct
• Xl-fli, early in pronephros and branchial arches, later in developing vasculature and endocardium of the heart.
• goosecoid, head mesoderm
• gremlin: pronephric duct, neural crest, crest derivatives
• HNF1 beta / LFB3; pronephros and endoderm
• HoxB9 (=XlHbox6), spinal chord. Note, only neural specific at tailbud stages. At least at early and midneurula, there is a significant amount of expression in the dorsal mesoderm (more than HoxD1)
• HoxD1, posterior neurectoderm.
• int-2, blastopore and hindbrain.
• xlHbox6; posterior spinal chord
• Xlim-1; some neurons, pronephros
• Mad; crest
• XMHC-alpha; heart
• Mix; early mesoderm and endoderm
• XMyf-5; dorsolateral marginal zone, somite
• XMyoD; lateral marginal zone, somite
• noggin, dorsal marginal zone, dorsal lip, prechordal plate, notochord.
• Xnot; organizer and presumptive notochord
• xNkx-2.5; presumptive heart and head endoderm fromstg 12 onwards
• N-myc. Anterior CNS and crest. Low levels in somites and pronephros.
• xnr3; superficial layer of organizer
• Xpat; germ plasm and primordial germ cells
• XPax-2; mid-hindbrain junction, pronephros, rectal diverticulum, pharyngeal aches, otic vesicle, spinal chord
• XPax-8; presumptive pronephros, presumptive otic vesicle.
• plaktoglobin; desmosomal-cadherin cell junctions
• Slug, pre- and post-migratory cranial and trunk crest.
• XSMP-30 (senescence marker protein -30); pronephric tubule specific
• shh, sonic hedgehog, notochord, thyroid, floor plate, visceral arches, ventral brain
• sybII, CNS.
• XTin1; presumptive heart and head endoderm
• xTwist; neural crest and lateral plate
• XVent1 and XVent2; ventral (Vent1) and ventrolateral marginal zone (XVent2)
• xWT (Wilms'); pronephric splanchnic mesoderm, later restricetd to glomus.
• Xwnt4, floor plate and part of pronephros.
• xwnt8; ventero/lateral mesoderm. Also see xwnt8 double stain with xnr3