Methods index

• detection of apoptosis via TUNEL
• detection of apoptosis via ELISA
• Lance Davidson's fluorescent in situ page
• TUNEL staining of apoptosis patterns
• transgenic Xenopus laevis methods- the breakthrough!
• Xenopus tropicalis- fantastic new system for transgenics, genetics, and hopefully soon (?), insertional mutagenesis
• listing of Xenopus cDNA and genomic libraries
• making affinity columns
• affinity purification of antibodies
• antibody staining protocol
• development substrates and stains
• fixatives
• in situ hybridization
• Modified antisense oligonucleotides for Xenopus research
• sectioning stained samples
• synthesizing RNA hybridization probes (digoxygenin and fluorescein tagged)
• recipes
• reagents
• suppliers. Comprehensive list with phone and fax numbers.
• antibody production services
• peroxidase blood staining method
• list of primers for RT-PCR from Guenter Lepperdinger