cyclin D1; eye, neyral plate
cyclin D1, nucleic acid
Photos and text by Matt Cockerill.
The following series of photographs of in situ hybridized embryos are a
compilation of two separate in situ hybridization experiments, using the same set
of fixed embryos. Both experiments produced very similar results, but the embryos
were bleached to different extents. Extensive bleaching allowed the signal to be
seen most clearly (bleaching was not found to cause significant leaching of the
product), but leaving some pigment unbleached had the advantage of making the
detailed morphology of the embryos more apparent.
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Figs A to D. Gastrula, diffuse cyclin D1 staining in whole-mounts is seen in the vegetal
hemisphere of the embryo, in an arc spanning somewhat more than 120*
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Figs E to H. At the end of gastrulation and the onset of neurulation, cyclin
D1 staining is more restricted, appearing in a wedge at the extreme anterior of
the embryo, at the anterior edge of the region which will become the neural
plate. Some weaker staining is also seen in regions slightly further dorsal and
posterior. E and G are dorsal views (ant to left), F and H are frontal, with dorsal up.
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In later neurula stages (figure i-l), staining is seen in a bilaterally
symmetric pattern on either side of the neural plate, with a distinct
sub-division into a lower and an upper band of staining. Simultaneous staining
with further molecular markers would be necessary to establish for certain
whether these regions of staining correspond to midbrain or forebrain. Further
staining is also visible at this stage extending along the neural tube which is
in the process of formation (figure 7.3 i,k).
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By stage 25-26 (very late neurula, just pre-tailbud, figure m,n), the
localisation of the cyclin D1 mRNA shows striking localisation to a subset of
anterior cells, some of the main regions of staining being: *Embryonic eye primordia *Neural crest *Anterior neural tube (strong staining) *Posterior neural tube (weak staining) *Tailbud.
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Comments and probe requests to Matt Cockerill
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