eFGF; marginal zone, blastopore
eFGF, nucleic acid
Cloned by Harry Isaacs in Jonathan Slack's lab. Zygotic eFGF expression is first detected in a complete ring around the blastopore at the start of gastrulation. At this stage there is a clear dorsal to ventral gradient of expression.
Whole-mount in situ hybridisation analysis of Xenopus eFGF expression during normal development.
Abbreviations: arc=archenteron. bp=blastopore. cnh=chordoneural hinge. dl=dorsal lip. dmz=dorsal marginal zone. mhj=midbrain/hindbrain junction. pw=posterior wall of the neuroenteric canal. som=somite. tlb=tailbud. vmz=ventral marginal zone.
A is a vegetal view of an early gastrula stage 10.5 embryo (dorsal to the top).
B is a vegetal view of a cleared early gastrula stage 10.5 embryo (dorsal to the top).
C shows the cut surface of a stage 10.5 embryo that has been dissected along the dorsoventral midline (dorsal to the right).
D is a vegetal view of a gastrula stage 11 embryo (dorsal to the top).
E is a vegetal view of a gastrula stage 11.5 embryo (dorsal to the top).
F shows the cut surface of a gastrula stage 11.5 embryo that has been dissected along the dorsoventral midline (dorsal to the right).
G is a dorsal view of a late gastrula/early neurula stage 13 embryo (anterior is to the left).
H shows the cut surface of a stage 13 embryo that has been dissected at right angles to the anteroposterior axis (transverse section) (dorsal to the top).
I is a posterior view of a stage 13 embryo (dorsal to the top).
J is a dorsal view of a neurula stage 14 embryo (anterior to the left).
K is a posterior view of a cleared late neurula stage 20 embryo (dorsal to the top).
L is a side view of the head region of a tailbud stage 32 embryo (anterior to the left).
M is a side view of the head region of a cleared stage 32 embryo (anterior to the right, dorsal to the top).
N is a side view of the posterior region of a stage 32 embryo (anterior to the left, dorsal to the top).
O is a side view of the posterior region of a stage 32 embryo (anterior to the left, dorsal to the top).