Xpat, primordial germ cells
Xpat (nucleic acid)
Xpat, or Xenopus primordial germ cell associated transcript. Cloned and Characterized by Clare Hudson and Hugh Woodland. As the name implies, a transcript initially localized to the vegetal pole, and later localized to the primordial germ cells.
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A. Cleavage stage, vegetal view. Staining has a granular appearance in a small disc at the vegetal pole. Earlier staining at the vegetal pole is in a flat disc which is not granular (Hudson and Woodland, 1998)
B. Stage 26. Cells containing Xpat have moved into the posterior endoderm, and remain there until around stage 35.
C. Stage 37. Xpat positive cells have moved anteriorly and dorsally and are in the process of settling in the genital ridges
Figures are Copyright © 1998, Elsevier Science Ireland Ltd.
- Reference; Hudson, C. and Woodland, H.R. Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis.
- marker name; Xpat
- plasmid; probe plasmid contains 2 kb of 3' UTR in Bluescript (KS)
- For antisense transcripts; EcoRI and T7
- Available from; Hugh Woodland
- Optimized conditions; .
- Other species; .
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