Plaktoglobin
Plaktoglobins are desomsomal-cadherin associated proteins involved in cell adhesion. The images shown here were plaktoglobin-GFP fusion proteins detected using confocal microscopy. They illustrate the subcellular distribution of various truncated forms of plaktoglobin protein. All images submitted by Mike Klymkowski.
Nuclear localization of N deletions. Fertilized eggs were injected with RNA encoding either ND2 (A), ND7 (B) or Arm5-8 (C) mutant forms of HPkg. Living embryos were examined by confocal microscopy at stage 10-12. In the ND2 expressing embryo, the bulk of the polypeptide appears in the nucleus, although cortical polypeptide can be seen. The ND7 polypeptide appeared to localized more equally to both nuclei and cortex. The Arm5-8 polypeptide was localized primarily to the cortex, although weak nuclear localization was still apparent. Scale bars mark 10mm in each part.
Image copyright 1997 - Developmental Genetics / Mike Klymkowsky
Ectopic expression of plaktoglobin causes axial duplications.
For more information visit the Klymkowski lab web site.
References;
Karnovsky, A. & M.W. Klymkowsky. 1995. Anterior axis duplication in Xenopus induced by the over-expression of the cadherin-binding proteins plakoglobin. Proc. Natl. Acad. Sci. USA ., 92: 4522-4526.
Kofron, M., A. Spagnuolo, M.W. Klymkowsky, C. Wylie & J. Heasman. 1997. Catenins have distinct functions in the early Xenopus embryo. Development, in press.
Adam Rubenstein, J. Merriam & M.W. Klymkowsky. 1997. Localizing the adhesive and signaling functions of plakoglobin. Developmental Genetics, in press.
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