Antibody production

For an online listing of antibody production services visit The Antibody Resource Page
The monoclonal production laboratory recommended by Jackson labs was Maine Biotechnology Services

1-800-925-9476
1037R Forest Ave.
Portland, Maine 04103
www.mainebiotechnology.com
They require 3 to 5 mg protein. 5 mice used, boost every 3 weeks, wait for high titre, rest best mouse 1 month, fusion, 4-7 plates of hybridomas. Retest all positives and supply supernatent from all positives. Generate 3 subclones for lines positive make ascites 6 to 9 months to ascites + frozen cells.

$7,500 can be made in three equal payments.


Pocono Farms. A fantastic service. A very professional and reasonably priced antibody production service. We have used Pocono to make antibodies against bacterial fusion proteins (Mal/Wilms' tumor). They supply you with serum from injected animals by FedEx at monthly intervals. They do mice, rats, rabbits, goats, guinea pigs sheep and chickens. They may also have a anti-peptide service these days.

Pocono Rabbit Farm & Laboratory, Inc. - Custom Antibody Production Services antibody@prfal.com - reaches all staff Phone 800-622-6381 or +1-717-595-7108 Fax 717-595-9277

Prices( per animal), average project rabbit project $312, chicken $184, guinea pig $194, mouse/rat $163. (primary injection plus three boosts and bleeds)


Green Mountain custom monoclonal production

www.greenmoab.com


Biodesign
A company producing both custom mono- ($8,500) and polyclonal antibodies. They have an extensive web site describing their various services. They also sell hundreds of immunological reagents.
Research Genetics (poly and monoclonal)

We have used this service for a number of polyclonal anti-peptide antibodies. Price is about $1195 to synthesize a ten-mer and immunize, boost and bleed two rabbits. Enough peptide is supplied to generate a couple of affinity columns. All of the antibodies produced recognize the appropriate peptides (tested by the company by ELISA). None of ours have worked on fixed embryos to date. They also make monoclonals in 24 weeks, $8,500. USA ph 800 533 4363 fax 205 536 9016 UK ph 0 800 89 1393


Alpha Diagnostic Intl. Inc. http://www.4adi.com
Note: the following information is from Alpha Diagnostics. I have no information from users of this service, but it sounds good!
Guaranteed custom services. If you supply an antigen to them (protein or peptide) and if you do not good titer antibodies, ADI will either credit the whole charges or do the project in another species. ADI also provides custom peptide and antibody (polyclonal and monoclonal) services.
BioWorld, ph 800 860 9729 or 614 798 9158, fax 614 798 9169 e mail info@bio-world.com
Cheap mono and poly service. We have not used their services yet.
polyclonal production (90 day schedule) $295 per animal
ascites production $23 per mouse (!)
monoclonal production;
immunization (6 mice) and testing $345
fusion and development $1495
screening (you could do this yourself) $600
cloning selected hybrids $295
expansion of slected clones $385

Animal Pharm Services. USA 800 808 0550; 707 431 0171.

Any information on quality of service?

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