Recipes

AP buffer; 100 mM Tris.HCl pH9.5, 50 mM MgCl2, 100 mM NaCl, 0.1% tween 20, 5 mM levamisole
BB:BA (Murray); 2 parts benzyl benzoate plus one part benzyl alcohol
bleach; 70 % methanol, 10% (final) hydrogen peroxide
CMFM (Sargent); 88 mM NaCl, 1 mM KCl, 2.4 mM NaHCO3, 7.5 mM Tris.HCl pH7.6
Holtfreter's Solution (1 X); 60 mM NaCl, 0.6 mM KCl, 0.9 mM CaCl2, 0.2 mM NaHCO3 Use at 0.1 to 0.2 x for embryo culture, at 1 x for dissections.
in situ hybridization buffer (Harland); 50% (v/v) formamide, 5 x SSC, 1 mg/ml torula RNA, 100 ug/ml herring sperm DNA, 1 x Denhardt's, 0.1% tween 20, 5 mM EDTA
NAM (Slack); (1 X)110 mM NaCl, 2 mM KCl, 1 mM CaCl2, 1 mM MgSO4, 0.1 mM EDTA, 2 mM Na phospahe pH 7.5,1 mM NaHCO3, 25 ug/ml Gentamycin
MMR; 1 X MMR is 100 mM NaCl, 2 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 5 mM HEPES, pH7.4. Use at 0.1 to 0.2 x for embryo culture, at 1 x for dissections.
RNA polymerase buffer (Krieg); (5 X) 200 mM Tris.HCl pH7.5, 30 mM MgCl2, 20 mM Spemidine
TBS; Tris buffered saline; 20 mM Tris pH 7.5 , 150 mM NaCl
TBST; TBS plus 0.05% tween 20