12/101; somite, muscle

Monoclonal 12/101; Somite

Generated and characterized by Chris Kintner and Jeremy Brockes. Somite specific in early stages, somite and other muscles in later stages. Does not stain heart.

Stage 30 lateral view

Here is a dorsal view of a stage 30 embryo

Search PubMed for papers by Chris Kintner
or for other papers on Xenopus 12/101

Technical notes

Reference; Kintner, C. and Brockes, J.P. (1984) Monoclonal antibodies identify blastemal cells derived from differentiating muscle in newt limb regeneration. Nature 308; 67-69.
Antibody name; 12/101
Raised in; mouse, monoclonal
Subtype; IgG
antigen; Newt regenerating blastema
Available as; ascites or supernatent
Available from; DSHB
Stock concentration; ascites, approx 2,000 x
Optimized conditions;
Other species; works on urodeles

all of these images are 100% digital, and were captured directly from the microscope by a NeXTDimension computer system

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