Cardiac Actin; muscle, heart

Cardiac Actin, nucleic acid

Cloned by Mohun et al., 1984. An abundant mRNA expressed in all muscle, including somite and heart. Staining and photos by Peter Vize.

Stage 34 embryo, lateral view. NBT/BCIP substrate.

Here is a close up to show the heart staining.

In situs can even be done with less sensitive development reagents, here is an example of a stage 34 embryo developed with the red AP substrate

Search PubMed for papers by Tim Mohun
or for other papers on Xenopus cardiac actin

Original Reference; Mohun TJ. Brennan S. Dathan N. Fairman S. Gurdon JB. (1984) Cell type-specific activation of actin genes in the early amphibian embryo. Nature. 311:716-721. Full length cDNA Stutz,F. and Spohr,G.Isolation and characterization of sarcomeric actin genes expressed in Xenopus laevis embryos. J. Mol. Biol. 187 (3), 349-361 (1986)
Recent application reference;
Data base accession number; Genbank XLACACR
Download DNA sequence
Plasmid name; pAC100
Diagnostic cut (to check identity of plasmid); EcoRI plus HindIII releases insert
For sense RNA transcripts cut with and transcribe with
For antisense RNA transcripts cut with PvuII and transcribe with SP6
Available from; Tim Mohun, NIMR, The Ridgeway,MillHill,London NW7 1AA (UK) or me
Average development time; 1 to 2 hours
Reactivity in other species; probably
all of these images are 100% digital, and were captured directly from the microscope by a NeXTDimension computer system
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