XMyoD
XMyoD, nucleic acid and monoclonal antibody
Cloned by Nick Hopwood et al. in the Gurdon laboratory. Initially expressed in both the lateral and ventral marginal zone (stage 10), but then becomes restricted to the lateral MZ by stage 12 (Frank and Harland, 1991). This image is from Dosch et al., 1997. A monoclonal antibody is available from John Gurdon.
Left image, stage 10+ vegetal view, dorsal is up; center image, stage 12, dorsal view, anterior is left; right image, stage 30
Figure is Copyright © 1997, Company of Biologists.
- References;
- Gene cloning; Hopwood ND, Pluck A, Gurdon JB. MyoD expression in the forming somites is an early response to mesoderm induction in Xenopus embryos. EMBO J 1989 Nov;8(11):3409-3417
- Antibody; Hopwood ND, Pluck A, Gurdon JB, Dilworth SM. Expression of XMyoD protein in early Xenopus laevis embryos. Development 1992 Jan;114(1):31-38
- Transient expression in ventral marginal zone; Frank D, Harland RM. Transient expression of XMyoD in non-somitic mesoderm of Xenopus gastrulae. Development 1991 Dec;113(4):1387-1393
- Activation of muscle genes by ectopic XMyoD; Hopwood ND, Gurdon JB. Activation of muscle genes without myogenesis by ectopic expression of MyoD in frog embryo cells. Nature. 1990 Sep 13;347(6289):197-200
- These images and excellent use as a marker; Dosch, Volker Gawantka, Hajo Delius, Claudia Blumenstock and Christof Niehrs. Bmp-4 acts as a morphogen in dorsoventral mesoderm patterning in Xenopus. (download abstract)
Development 124, 2325-2334 (1997)
- Available from; Christof Niehrs
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