Wilms' tumor gene WT1; pronephros, glomus
Xenopus Wilms' tumor gene.
Cloned and characterized by Peter Vize and Tom Carroll, UT Austin.
Stains the pronephric splanchnic mesoderm from stage 20. This will form the glomus of the pronephros around stage 30. Two figures illustrating the anatomy of the glomus are available, one by Carroll and Vize (1996), and one by Balinsky (1970). Note that the glomus is not the pronephros, it is a seperate vascularized structure which is a part of the functioning pronephric unit. xWT1 is therefore a marker of the glomus, not of the pronephros. To view some histology illustrating glomus structure see the glomus page.
A. 
B.
A. Stage 24, Lat, blue.
B. Stage 30, Lat, blue.
C. 
D. 
C. Stage 39, lateral view. Expression in the glomus of the pronephros, no expression in pronephric tubules or pronephric duct.
D. Transverse section through WT stained embryo The stain is restricted to the splanchnic mesoderm which will form the vascularized portion of the pronephros, the glomus.
- Original Reference; Carroll, T.J. and Vize, P.D. (1996) Developmental Dynamics, 206, 131-138. . Abstract. Entrez.
- GenBank accession number; U4Z011
- Plasmid name; pxWT1-gem. 1.2 kb cDNA in BamHI site of pGEM7
- Diagnostic cut (to check identity of plasmid); BamHI to release insert of 1.2 kb
- For sense RNA transcripts cut with XhoI and transcribe with SP6
- For antisense RNA transcripts cut with SacI and transcribe with T7
- Available from; Peter Vize
- Stock concentration; 20 ug/ml in TE
- Optimized conditions; standard
- Average development time; 5 hours or more in BM purple. It is low abundance so it takes a while
- Reactivity in other species; unknown
Peter Vize
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B.
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