Xlim-1; dorsal lip, pronephros, nervous system
Xlim-1, nucleic acid
Cloned by Masanori Taira et al., in Igor Dawid's lab at the NICHD (1992). Expression in the pronephros and CNS described by Taira et al., 1993. A homeobox and lim domain gene required for axial patterning and kidney development in mice. Ectopic expression of mutant Xlim-1 induces secondary axes in Xenopus (Taira et al., 1994b). Early expression in dorsal lip and notochord, later expression in pronephros and CNS.
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A. Ventrolateral view (anterior to the left) of a stage 14 embryo, expression is in a broad stripe in the lateral mesoderm.
B. Stage 17/18 neurula (dorsal view, anterior to the left). Expression in the neural tube, and in the presumptive pronephros (black arrow).
C. Lateral view of stage 26 tailbud. Expression in pronephros, CNS and tailbud.
These three figures are Copyright © Company of Biologists, 1993.
- References; Taira, M., Otani, H., Jamrich, M and Dawid, I.B. (1994). Expression of the LIM class homeobox gene Xlim-1 in pronephros and CNS cell lineages of Xenopus embryos is affected by retinoic acid and exogastrulation. Development 120, 1525-1536.
Taira, M., Jamrich, M., Good, P. and Dawid, I.B. (1993) The LIM domain-containing homeobox gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos. Genes & Devl. 6, 356-366.
Taira M. Otani H. Saint-Jeannet JP. Dawid IB. (1994b) Role of the LIM class homeodomain protein Xlim-1 in neural and muscle induction by the Spemann organizer in Xenopus. Nature 372: 677-679 [published erratum appears in Nature 1995 Feb 2;373(6513):451].
- Plasmid; pXH32
- Construction; 1.35 kb EcoRI insert in pBlueScript KS (+)
- For antisense probe cut with XhoI and transcribe with T7
- Download Sequence
- Available from Igor Dawid
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