Transgenic frogs

Transgenic Xenopus

Technology developed by Enrique Amaya and Kris Kroll.

GFP expression in transgenic tadpole driven by gamma-crystallin promoter
photos courtesy of Enrique Amaya! Additional photographs of transgenic expression available on Enrique's web site:

Cardiac actin promoter driving expression of GFP in transgenic somite and heart
Cardiac actin promoter driving expression of GFP in heart and head muscle
Neural specific beta-tubulin promoter driving CAT expression. Comparison between plasmid expression and transgenic neurulae.
Axons extending from the olfactory placode to the olfactory bulb
Trouble shooting and technical tips for transgenic production

Technical information and movies demonstrating this technology will be available here soon!

References:

Amaya, E. and Kroll, K.K. (1996) A method for generating transgenic frog embryos. in Methods in Molecular Biology, Vol. 3X: Molecular Embryology: Methods and Protocols. Edited by Paul Sharpe and Ivor Mason. Humana Press Inc., Totowa, N.J., in press.

Kroll, K.K. and Amaya, E. (1996) Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation. Development 122:3173-3183.

Link to Enrique's web site

[ XMMR Marker Index | Search XMMR and Xenbase | XMMR Welcome Page ]
[ The Virtual Library | The Virtual Library BioSciences database ]