Antisense News
Those interested in studying the function of maternal or early zygotic genes may like to try a different antisense approach. Morpholino oligos (marketed by Gene Tools) are less toxic than phosphorothioates, and can be injected into fertilized eggs. In recent experiments targetting maternal beta catenin, a morpholino caused a ventralized phenotype when injected at the two cell stage. It also continued to affect beta catenin protein levels through the neurula stage. For more information contact:
Janet.Heasman@chmcc.org