Genetics of Amphibian Development: Moving Into Xenopus tropicalis

University of Virginia, Charlottesville VA,

Saturday, June 12, 1999 9 a.m. - 6 p.m.

Sunday, June 13 11a.m.-5p.m.
preceding the SDB annual meeting June 13-18

This meeting will focus on building a genetics community to complement embryological and molecular research in Xenopus development. Presentations will center on laboratory use of the more genetically-malleable Xenopus tropicalis, and will include discussion of related issues, e.g., analysis and manipulation of ploidy in Xenopus, and recent advances in transgenesis. Special emphasis will be placed on learning from the zebrafish community's experience. Following the presentations, there will be an open caucus to discuss generating shared genetic research infrastructure, such as genetic and physical maps, stock centers, and mutagenesis strategies. Attendees are encouraged to present posters speculating on how they would like to use specific genetic strategies. On Sunday, June 13, there will be a tour of the X. tropicalis facility at U.Va. and a transgenesis workshop. For more information or to register, email xenopus@virginia.edu or visit the X. tropicalis website at http://minerva.acc.Virginia.EDU/~develbio/trop/.

Program

9:00 a.m.-12:30 p.m.
Coffee

Introduction to Xenopus tropicalis
Rob Grainger, Univ. of Virginia

Review: Genome Manipulations and Mutants in Amphibians
Darcy Kelley, Columbia Univ

Genetic Screens for Vertebrate Developmental Genes
Mary Mullins, Univ. of Penn.

Contrasts Between X. tropicalis and X. laevis Major Histocompatibility Complex: the Influence of Polyploidy on Gene Silencing
Martin Flajnik, Univ. of Maryland

Embryology of X. tropicalis
Ray Keller, Univ. of Virginia

Laboratory Husbandry of X. tropicalis
Nick Hirsch, Univ. of Virginia

12:30 p.m.
Lunch and posters

Session II: New Approaches To Manipulating Amphibian Genomes

Transgenesis in Xenopus: Present and Future
Enrique Amaya, Wellcome/CRC Inst.

'Gene Trap' Insertional Mutagenesis of X.tropicalis
Odile Bronchain, Wellcome/CRC Inst.

Strategies for Inducible Control of Gene Expression in X. tropicalis
Lyle Zimmerman, Univ. of Virginia

Transgenic Analysis of Opsin Promoter and Function
Barry Knox, SUNY Syracuse

Saturation of Zebrafish Notochord Mutations: Can new loci be identified in Xenopus tropicalis?
Derek Stemple, NIMR, Mill Hill

Refreshments

6:00-12:00
Shuttle bus every half hour to/from evening social

Sunday, June 13
11:00 a.m. Coffee
11:30 a.m. X. tropicalis facility tour
1p.m.-5p.m. X. tropicalis transgenesis workshop

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