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Fig. 10. Quantification of pyknotic nuclei and apoptotic cell death in Psoralen only (whole cornea), UV only (whole cornea), and UV only (half cornea) controls. (A) Similar to the untreated control corneas (black line), both the Psoralen only controls (blue line) and the UV only controls (red line) showed minimal levels of pyknotic cell death at all time points examined. (B) Compared to the untreated control corneas (black line), the average percentage of TUNEL positive nuclei was lower in both the Psoralen only (blue line) and UV only (red line) controls at all time points. (C, D) At each time point, both the untreated halves (blue lines) and the UV only treated halves (red lines) of the corneas showed similar minimal levels of pyknosis (C), and apoptosis (D). The untreated controls (whole cornea) from (A, B) have been included in (C, D), respectively, for convenience of comparison. Error bars represent standard error of the mean. (B, D) Boxes represent lower and upper quartiles; whiskers and outliers represent Tukey range. Statistical analysis has been done for control (whole cornea) vs. Psoralen only treated, and control (whole cornea) vs. UV only treated in (A, B), and untreated halves vs. the UV only treated halves of the corneas in (C, D). Outliers have been included in calculating p-values in (B, D). N = 6-7 for Psoralen only, UV only, and UV only half treated corneas at all time points in (A–D). N = 9 for control (whole cornea) for all time points in (A–D). * = p ≤ 0.05 (displayed for UV only). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) Image published in: Adil MT et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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