|
XB-CLONE-2479649
| Clone Name: IMAGE:8889163 | Gene: fth1 | Species: Xenopus tropicalis | |
Sequence:
5' EST: EL847740 
[+]
Library:
| Name: NICHD_XGC_trop_25 | External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library |
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.
| Anatomy: whole organism | Stage: NF stage 25 |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map | Name: pCS107 Type: plasmid 5' Restriction Site: SalI 3' Restriction Site: NotI |  |
Usage:
Suppliers:
Search for clone at: Open Biosystems