XB-CLONE-2589439
Clone Name: IMAGE:8923973
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Gene:
rprm
,
fam3b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154872 [+]
CGCTGCTGGGGCTGCTGTGCGCCAGGGGAGTGTAAAGCGCTGTCCAGACTGAGAGAATGGTCAACTTACGGTTCCTGTCTAACAACACGGTGAAGGTGGT
CGGCCTGTTATGTGCCTCTGCGTGTGCCTGGTACATGGGATACCTGTTTGCGGAGGTATTGCCTGAGGAACCATGGCAGGCAGCAGTTGGAAGCATGCAA
AGCCTAGCGCAGAAACCCGTACTGAAAGCCCCCAATCCAAAAAGGCAGAAATGTGACATCTGGGTGTCCTGTGCGCCAAACCAGCTGGCATATCGCATAC
GGACTGGCGGAGCGAAAGACATCATGCCTGAAGTTTGTTTGGATGATAACATGCTCATCACAGGAAAGAATTCCAACCGAGGCATAAACATTGCTGTTAT
TAGCTAGACCTGGAAGCTCGTTGAGACGAAGACGTTTGACATGTATGAAGGAGATTTTTCAGGCCCTATGATTGAATACCTTAACAAGATACCAAAAGGG
GCTCTCGTCATGGTCGCCACTCACGATGACGCAGCTACCAAGTTGAACGACCCAGCGAAAAAAGCTTTTGAAGACCTCGGGAGCAAGGAAGTTCGAAATC
TTCGTTTCCGGTCAGCCTGGGCGTTTATAAGCTTTAAAGGATCTCAAATACCGGACAACATTGAAAAAGAAAAGATCAACCACTCAGACGGAAGCAAGAA
CAGATATTCCGGTTGGCCGGCAGAAATCCAGATTGACGGCTGTCTCCCCAAACCATAAACAAGCCCCATTGGGTCAAGCCGGCAGGAATCCATCCTCTCT
TCCAATGGAGGCAGAGCATATGGAGCGAATAAAAGGACCGGATTGCCGAAGGGAACCTGCCCACCAACTACTAATGATTTTGAACAACTGGCTGAAACCA
ATATGGCATTTAATTTATATAAATTTATAGAAAGAGGCCGACGTGCTTCTGTGCCGAATACCACCAAATATATGTATGTGCCCCCTTACGGGCTTGTTTC
ATACACTCCATTCCATCATCACTGGAGCCGGGAATCATCTCCGTGGTGACCGTTGTCCCGTTGATTCTGATCCGGTAGAAGATCAGGAAACTGGTGCTCT
CAAATACAGACTGACACAACATCCAGGGTTTTTAGGCTGTAAATAATGGTGTTTTGGTTATGTTCCATAGCACAGAGATAATGATTATTCACTATAGGGT
TTGGCTGTGGGATTCCTATGGTCACATGGGGAGAGGAAGACTCTGTGTGACCCTTAATGATCCGGCAAAAGCGATTGCCCATTTCCTCCCTTGTGGTGCA
TCAGTAAGGCTTAGGGATACATGGCGCTTCCACTGGCGCTATTTTACCACCGTCAGACTGACATTGGGATTTGCACAAAGCTGTGACTTTTTTTCATACA
AATCCCATTACAGGGTGGTGTATCCATCTGTCCAATGCCAAGCGTCAATTCCTCCAGCCTTCAGATTTGCTAAGTGTTGCACTACAGCTTATTTAAAGGG
GACATTTATTCTAAAATAAATATTTTGTTATAATGCCAGTACAGGTATTGGACCCCTTATCCGGAAACCCATTATCCAGAAAGTTCCGAATTACAGAAAG
GCCATCTCCCATAGACTCCATTATGATCAAATAATTCACATTTTTAAAAATGGTTTCCTTTTTCTCTGAAATAATAAAACAGTACCTGTACTTGATTCCA
ACTAAGATATAATTACCCCTTATTGGGGCAGAACAGCCCTATTGGGTTTATTTCATGGTTAAATGATTCCTTTTTCTCTGTAATAATAAAACAGTACCTG
TACTTGATCCCAACTAAGATATAATTACCCCTTATTGGGGCAGAACAGCCCTATTGGGTTTATTTAATGGTTAAATGATTCCCTTTTCTCTGTAATAATA
AAACAGTACCTGTACTTGATCCCAACTAAGATATAATTACCCCTTATTGGGGGCAGAACAGCCCTATTGGGTTTATTTAATGGTTAAATGATTCCCTTTT
CTCTGTAATAATAAAACAGTACCTGTACTTGATCCCAACTAAGATATAATTACCCCTTATTGGGGGCAGAACAGCCCTATTGGGTTTATTTAATGGTTAA
ATGATTCCCTTTTCTCTGTAATAATAAAACAGTACCTGTACTTGATCCCAACTAAGATATAATTACCCCTTATTGGGGGCAGAACAGCCCTATTGGGTTT
AATTACTGTTAAAATATTTTTTTTAGTACCCTAAAGGTAGGAGATCCGAATTACAGAAAGACCCCTTATCTTAAAAAAACACCAGGTCCCAAGTATTCTA
GATTACGGGTCCCATACCTGTACTAGCATTTACTTTTAAGAATTGTTATTTAGCAAACTGGCATTTAATAAGACTTCAATCAAAAGAGAAGACCTGTATT
TGTGGCTGAAAAAAGTGTTATTTAGTAAAAATAATGTAGGAATTGGGGGAAAGGGTTCACTTAAAGTCACTCTATGCTTTTTAGGAAACTCTTTAAGAAA
TCTTTGGTACATAAAAATGAAATAAATGTTTTTTTTTCTAAAAAAAAAAAAAAA
5' EST: EL669047 [+]cgctgctggggctgctgtgcgccaggggagtgtaaagcgctgtccagactgagagaatggtcaacttacggttcctgtctaacaacacggtgaaggtggt
cggcctgttatgtgcctctgcgtgtgcctggtacatgggatacctgtttgcggaggtattgcctgaggaaccatggcaggcagcagttggaagcatgcaa
agcctagcgcagaaacccgtactgaaagcccccaatccaaaaaggcagaaatgtgacatctgggtgtcctgtgcgccaaaccagctggcatatcgcatac
ggactggcggagcgaaagacatcatgcctgaagtttgtttggatgataacatgctcatcacaggaaagaattccaaccgaggcataaacattgctgttat
tagctagacctggaagctcgttgagacgaagacgtttgacatgtatgaaggagatttttcaggccctatgattgaataccttaacaagataccaaaaggg
gctctcgtcatggtcgccactcacgatgacgcagctaccaagttgaacgacccagcgaaaaaagcttttgaagacctcgggagcaaggaagttcgaaatc
ttcgtttccggtcagcctgggcgtttataagctttaaaggatctcaaataccggacaacattgaaaaagaaaagatcaaccactcagacggaagcaagaa
cagatattccggttggccggcagagatccagattgacggctgtctccccaaaccatatacaagccccattgtgtcaagccggcaggaatccatcctctct
ttccatggaggcagagcatatggagcgaa
3' EST: EL669046 [+]agaaaaaaaaacatttatttcatttttatgtaccaaagatttcttaaagagtttcctaaaaagcatagagtgactttaagtgaaccctttcccccaattc
ctacattatttttactaaataacacttttttcagccacaaatacaggtcttctcttttgattgaagtcttattaaatgccagtttgctaaataacaattc
ttaaaagtaaatgctagtacaggtatgggacccgtaatctagaatacttgggacctggtgtttttttaagataaggggtctttctgtaattcggatctcc
tacctttagggtactaaaaaaaatattttaacagtaattaaacccaatagggctgttctgcccccaataaggggtaattatatcttagttgggatcaagt
acaggtactgttttattattacagagaaaagggaatcatttaaccattaaataaacccaatagggctgttctgcccccaataaggggtaattatatctta
gttgggatcaagtacaggtactgttttattattacagagaaaagggaatcatttaaccattaaataaacccaatagggctgttctgcccccaataagggg
taattatatcttagttgggatcaagtacaggtactgttttattattacagagaaaagggaatcatttaaccattaaataaacccaatagggctgttctgc
cccaataaggggtaattatatcttagttgggatcaagtacaggtactgttttattattacagagaaaaaggaatcatttaaccatgaaataaacccaata
gg
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: thymus | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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