XB-CLONE-2900381
Clone Name: CAAO7990
|
Gene:
XB985134
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX942012
[+]
tcagctgatctctgagggagcacacagcccatcactaaatggtttatatcttcagaggtcaagtgaggcaatgggcataatctgaggatgtcaacattgt
gggatttattttgctccaggaaaatgcatacaaaaagtaaagaaaagagagcaaatggaggaccggggaaaaatgaacctgaaaaaatgcagatgaagca
agaaatcacgctgtttaatggtgtttctttaattgttggaaatatgattggctctgggatatttgtatcccccaaaggagttcttatgtacagcacctca
ttcggactctctctcattatctgggcacttggaggaatgttttccatgttcggagctttgtgctacgcggagctgggtacctctgttattaaatccgggg
caagctatgcatatatccttgaagcctttggcggtttcattgccttcatcagactctggtcttccctgttaataatagaacccacaacacaggccattat
cgccattacgtttgcaaattatattgtgcagccaatttttctatcctgtcaaccaccttatgcagctgttcgactgattgcagcagcctgcatttgcacc
ttaacgtttattaactgtgtccacgtgagatgggggacgagagtacaagacgtgttcacctatgccaagataatggctctcattatgattatatcagtgg
gtctagctaaaattgtgcaaggtgaaagtgataatctaa
3' EST: CX942011
[+]tttttttttttttttcagatggcggaaatactttattatattaaacttggtacatacaatatatttactatgtgtaagtatacatcaccaggctgcctgc
agacaacaattagtgagaaaacaagaactattggttctgaaaccaagagctcaaagtcagaacaatgggatcagaataataacggtaatgctgagcaatg
catttcataaaacatttcagtttaaaggaacagcagtgctatatttacattacagttctctgcttttgatcataactatgctgagacgtaccatatatac
atcaacccaatgcaaaatagagtaaataaaagatatgtattatatatatacacagaatgaaatgattaaatacatcatgccttatatatagtgcaatttc
tcgatgtctgaaatgcagtttagaaatcccgcttagagtaagtgcattcatatttctttaatcgtcgtaaccttaacttttcttttcttcatcgagatca
gtcaggcagcaattgcacaagagctgtacatattttgttattgatgcttgacaggacgttccctttgaggctgagtgattcgtaaaactattaacccggc
gacagagagtcccacgaatagccagtaactgaaactataatagtttataagcagaaacacatcttccacaagtagatagaggatggctattagtccattg
aagagaagagctggcactggtgtaaagttctccacatgaatcaagctcagacttgcagggaggtggccttgtcttgctccaacatagaacagtcttga
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: