XB-CLONE-3058375
Clone Name: IMAGE:7847633
|
Gene:
pcmtd2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT531636 [+]
tttttttctatgaaaaaaaaatggctgtttatttacgttggacacaactacaaactagacaaatatatattatatttcacacacagttatagatttatat
aaatatattgaaaggaaagtaacagcctgagcgaggctccagtttcatgttacacctttattggggggttggaataggaatctgcttatggggggcagaa
cgagtgcctgtggggcatcaggttgctatagcaacgtcggaacatgaggggccggtggggtttggcccgtttcctacaggctgataaataaatgtcctag
gaaattccgagacccccccccccccccaggcccggacagggaatctgggggttctgactgctgtaagatgctttagacagtgactgtttattgggcccat
ggggggctgtttgggcccctgaaatgcctgttttgaagaacaatccggactgcccccccccctacaaaccataaataaggcacaacacagaatagcaggg
ggtcccccaaacattctagaccactgagccctcacaccggaaccccaggagtgggcggaacccagaattacaggactaagtgccggattttggaaggagg
ggcccaaaatgtcagtttatttcccttcaccaaagaatcacgcacaaactccaaaccagaaccagaaccagagggtaaagttactgtaaatgagtgaaac
aaagggaaatgctgagacagaaccatcactgttggctgctgggcccagtttccccag
3' EST: DT531635 [+]gaacgaacctcgggccaatcagctgcagcgatggggggagcggtgagcgccgggcaggacaacgatgagttaatcgacaacctgaaggaggctcagtaca
ttcgctcggaggcggtggaaagggctttccgggccatcgaccgctccgagtattacctggaggagttcaagcagaacgcgtaccgggacttggcctggaa
acacggcaacatccacctgtcggccccctgtatctactccgaggtgatggaggccctcgacctgcagccgggactgtccttcctaaacctgggcagcggc
acgggctacctgagcaccatggccggactcatcctcggaccctttggcaccaatcacggagtagaaatccacgccgacgtaatcgaatacgccaaggaga
agctggaccagtttgtgcgcaccagcgacagcttcgacagatttgatttctgtgaaccttcctttgttgccgggaactgcctggacatcgctctggacgc
tacgtacgaccgcgtgtactgcggcgccggggtgcagaaggagcacaaggagtatatgaagaacctgatcaaggtgggcgggatcctcgtgctgccattg
gaggaaaagctgaccaagatcactcgcacggggccctcctcctgggaaaccaaaaagatcctggccgtgtccttcgctcccctcatccaaccggaaacgg
ccgacgcctccaaggccaagaacctgcggctgccgctgcccaatatccgctccctgcaggatctcgcccgcatcactatcagggggcacattaaacggct
gataagccaagtgcccagcggccggggggagggggtgctgaggaaccccccgcgctacaagaggaagcggctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: