XB-CLONE-3176134
Clone Name: IMAGE:7854920
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Gene:
tnnt3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT397023 [+]
caggtgaccaaaagccatcatgtctgacacagaggaagttgaacaggtggaggaggagaagccaaaacccaaactgacagcccctaaaatcccagaggga
gaaaaagtagactttgatgatatccaaaagaaaagacaaaacaaagacttgattgaactacagtctctgattgacacacattttgaggcccgcaagaaag
aagaagaggagctcattgccctgaaagaaagaattgagaaacgcagagccgagagatctgatcagcagaggatcagagcagagaaagacaaggagcgtca
gaatagattggctgaagagaaggcaagaagagaggaacaggatgccatcaggcgtgcagaggatgacatgaagaagaagaaggctctgtcctccatgggt
gccacatacagcagctacctggctaaggctgatcagaagagaggcaagaagcagacagcccgtgagcaaaagaagaagattctggctgaccgtcgcaaac
cactgaatgtcgaccacatgaatgatgacaagctcagggaaaaggcaaaggaaatgtgggactggctgtaccagttggagtctgaaaagtttgagatggg
agagaagctgaagaaacagaaatacgagattacaaccctcaggaatcgtattgatcagctgcagaaacacagcaagaaggcagctggaggcaaaggaaaa
gttgggggccgctggaagtaaatcaactcgaaatcaacacccggtcctcacagagagcagcccctttctgccatttcttcagtactcgtttaggaagctg
gttccactccatgcaccctcaagggccatttcccttttgtgccaccaagttctctctgtggactgctttattatctcttc
3' EST: DT397022 [+]ttggggtaagaagcatcacactttatttacaaaagggaagagataataaagcagtccacagagagaacttggtggcacaaaagggaaatggcccttgagg
gtgcatggagtggaaccagcttcctaaacgagtactgaagaaatggcagaaaggggctgctctctgtgaggaccgggtgttgatttcgagttgatttact
tccagcggcccccaacttttcctttgcctccagctgccttcttgctgtgtttctgcagctgatcaatacgattcctgagggttgtaatctcgtatttctg
tttcttcagcttctctcccatctcaaacttttcagactccaactggtacagccagtcccacatttcctttgccttttccctgagcttgtcatcattcatg
tggtcgacattcagtggtttgcgacggtcagccagaatcttcttcttttgctcacgggctgtctgcttcttgcctctcttctgatcagccttagccaggt
agctgctgtatgtggcacccatggaggacagagccttcttcttcttcatgtcatcctctgcacgcctgatggcatcctgttcctctcttcttgccttctc
ttcagccaatctattctgacgctccttgtctttctctgctctgatcctctgctgatcagatctctcggctctgcgtttctcaattctttctttcagggca
atgagctcctcttcttctttcttgcgggcctcaaaatgtgtgtcaatcagagactgtagttcaatcaagtctttgttttgtcttttcttttggatatcat
caaagtctactttttctccctctgggatttaggggctgtcagtttgggttttggcttctcctcctcacctgttcaacttcctctgtgtcagac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: