XB-CLONE-3176717
Clone Name: IMAGE:7854913
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Gene:
gapdh
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396639 [+]
aatcaatggatttggccgcatcgggcgcctggtgacccgcgctgctgttttaagcggcaaatgtgaagtcgttgcaatcaatgaccccttcattgacttg
gactacatggcttacatgttcaaatatgactccacccacggccgattcaagggaacagtgtgtgttgagaatggcaagctggttatcaatggcaaggcag
tcaccgtcttccaagagcgtgacccctccaacattaagtggggcgatgctggtgcagtgtatgtggtggaatctactggagtcttcacaaccaaagagaa
ggccggtttgcacttgaagggaggtgccaagcgtgtcattatctctgccccctcagctgatgcccccatgtttgttgttggggtgaaccatgacaaatat
gataactctcttcacgttgtcagcaacgcatcctgcacaaccaactgcctggctcctcttgcaaaggtcatcaatgacaactttggcatcctggagggac
tgatgactaccgtccatgccttcactgccacccagaagactgtggatggcccatctggcaagctgtggagagatggcagaggtgccggtcagaacatcat
ccctgcctcaactggtgcagcaaaggctgttgggaaagtcatccctgagctgaatggcaaactgacgggaatggctttccgtgtccccaccccaaatgtc
tccgttgtggatctgacctgccgtctgtccaagcctgccaagtatgatgacatcaaggctgccattaagactgcagcacatggcccaatgaanggaatcc
tgggatacacagaagaccaggttgtctccactgacttcaatggtgacactcattcctccatctt
3' EST: DT396638 [+]gatgtttcattgttttatttagtaacacagaaacagtgacacagacaaactaccaggatgggatcgtgactctgggccccttcagtgacagagaggggtt
ggtggggcgacaggtaagtgcttattccttagaggccatgtgacacatgagatccacaacacggtggctgtagccacactcgttatcataccaggaaacc
agcttcacaaagttatcgttcagtgcaattccagctccagcatcaaagatggaggaatgagtgtcaccattgaagtcagtggagacaacctggtcttctg
tgtattccaggattcccttcattgggccatgtgctgcagtcttaatggcagccttgatgtcatcatacttggcaggcttggacagacggcaggtcagatc
cacaacggagacatttggggtggggacacggaaagccattcccgtcagtttgccattcagctcagggatgactttcccaacagcctttgctgcaccagtt
gaggcagggatgatgttctgaccggcacctctgccatctctccacagcttgccagatgggccatccacagtcttctgggtggcagtgaaggcatggacgg
tagtcatcagtccctccaggatgccaaagttgtcattgatgacctttgcaagaggagccaggcagttggttgtgcaggatgcgttgctgacaacgtgaag
agagttatcatatttgtcatggttcaccccaacaacaaacatgggggcatcagctgagggggcagagataatgacacgcttggcacctcccttcaagtgc
aaaccggccttctctttggttgtgaagactcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: