XB-CLONE-3177520
Clone Name: IMAGE:7854409
|
Gene:
phb1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT396108
[+]
tttgatttaacccgcaggaccaatggcagcacgactgtttgagactattgggaaacttggcctaggactggctgtcgctggaggggttgtgaactctgca
ctatataatgtggatgctggacaccaggcagtcatctttgataggttccgaggagtacaggaaacagtagtgggcgagggaacccatttccttattcctt
gggtccagaaacccatcattttcgactgtcgctcacgtcctcgcaacgtacctgttgttacagggagcaaagatctacagaacgtcaacattacactgcg
tatccttttccggccaatgggtaatcagctgccgcgaatcttcaccagtattggagaagattatgatgagagagtcctgccctccattactactgagatc
ctaaaatctgtagtggcccggttcgatgcaggggagctgatcacacagagagagctggtgtccaggcaggtgagcgaggatctcatggagagagcagcca
ccttcggcctcatcctggatgatgtgtcactgacacatctgacctttggcaaggagttcacagaggccgtagaagccaaacaggtggcccagcaggaagc
agagcgtgccaggttcattgtggagaaggctgagcagcaaaagaaagctgcagtgatttctgcagaaggggactccaaggcagcagagctcattgcgacg
tcactggcggatgcaggagatgggcttatcgagctgcgcanactggaggccgcagaggacatcgcgtaccagctgtctcgagctcgtaatgtcacctacc
ttccctcaggacagtcaacattattac
3' EST: DT396107
[+]gaaaatcctctgagggataacttttattgttagtgacagaaggccagattttaggtttagacataaacaagtgcttcaaccaaaaatgtattatgccctc
cattgaaaaaacaattatattcaattcaaattcagaaatatattggcaggattcaaacagttctgtggctaaagtccatttcatgtagggtattatgtgg
gctctgtaggagattcaccttcatacacaatcttctgtaacatttcccaagggaaattggccaattaacaaagacctatgtaggggtcagacgtaaaaaa
aaataaatggcatgacaacacatcatccaattactgtggtagctgtaataatgttgactgtcctgagggaaggtaggtgacattacgagctcgagacagc
tggtacgcgatgtcctctgcggcctccagtttgcgcagctcgataagcccatctcctgcatccgccagtgacgtcgcaatgagctctgctgccttggagt
ccccttctgcagaaatcactgcagctttcttttgctgctcagccttctccacaatgaacctggcacgctctgcttcctgctgggccacctgtttggcttc
tacggcctctgtgaactccttgccaaaggtcagatgtgtcagtgacacatcatccaggatgaggccgaaggtggctgctctctccatgagatcctcgctc
acctgcctggacaccagctctctctgtgtgatcagctcccctgcatcgaaccgggccactacagattttaggatctcagtagtaatggagggcaggactc
tctcatcataatcttctccaatactggtgaagattcgcggcag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: