XB-CLONE-3180779
Clone Name: IMAGE:7853395
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Gene:
inafm1
,
cactin
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393951 [+]
ctgagtctccctataacaagtgcttgcctgcctgaccctgtattcaacattcctaattgaagaaagctagtgctgtgtggccaagtcaatgttgttgtta
cagtctacagtatttttaattcagttctttttatatcaatgagttaaagcttatgttaaaggacatggcgactaatctccccgaaatgccatcccaccgg
caagaatgtaaatcgccggtgggatggcatacgcggcgcagtgatttgccaaagttgcggaagttgagaggaaacttccgcgacttcgagatctattgtg
aaatttttttatatttaattttgaagtttcacatggggctagccatattcttcatctcccagggtgccacaaccatgtgacctgtgctctgataaacttc
agtcacacttaactgctgagctgcaagttgaagtgatatcatcctcccaccaatcctttcccacccagcagctgatcagcagaacaataggaaggtagca
agatgggatggtggtagcagctcccagtagatatcagaatagcactcaatagtaagaaattcaagtctggctggggactcccccaggtacattggattag
gagaaacaatagatttgtttcagacttgggcacaatgcactaagatggctgcctacacacaaatattacaactaaagaataacattttaaatggcagagt
gaattatttgctatgttaacagtgtaatttagaaataatacatataccataaaaaccatgaccatattcctttaataaaggctatatctctgctgtaaag
cgctgcgtacataagtagcgctttataaataaagatatacatacatacatacatataatctcactgaaataaag
3' EST: DT393950 [+]ggtatttaaattccatgtttatttgtacaaaatattcccagaaaaatgcttttccattcacactgatgggaaggttagaaacaaaacagatgccactagt
tctacagaaagtaaattactaagagacatctggttcacagttctggagttcttccattattccaagatggaaaacaaaagataatagcaagcacatgttc
acactgcagttatcttctgtaacgatatcgcttgaagtggaaccacagctggaagataccatttgaaaactggcaacgaaaaccatgacggtgagaatac
tcccactctctgttgacaatcttaaaagctatgtcttcatatggaggcccagcatggaagcgtaggatggcaaagtctttgttgtcatggcaggcttcaa
gaaaatattctggagtagatctcttgtcaatcaagtcaggatagaaaatattgaatttatagccctgcacaattttgggaggagggttatcaaaatcata
atgggtctgattgtatttgttccattcaaaaccagtatgaactctgttgaagaaccgtggttttcggggtctgtacttgtcagcccaaaggtatgctttg
ctggtgactggaatctccacactaaattgagcttcatctccatccataccttcttttgccttccgtatganaatgtcttctgggctctcacttgcatctc
ctgtgacttgcagttgctgtctgcagagcaccgttctctgttggtcctcttcgaaatctactacatgagcatcaaaaggtagttcctgcactgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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