XB-CLONE-3186364
Clone Name: IMAGE:7851453
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Gene:
sae1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT390261
[+]
cttaagttataaagagctgtaggggaggatcacagaccaaggaagccttagtttgacagtaagttggaaatctcctgaaacacaagcattcccattgcat
tttgttttttgtccttgtcagaaagtacagttctgcccactaaaagatgccctagaaattgactggcatagtgaaaaggcaaagtctgctctgaagaaga
ctcccactgacttcttccttttgcaagttttaatgaagtttcgcacagacaagaaacgggacccccagccaagtaattaccaggaagactctgagctgct
gcttcagatctgcagtgacgttctagactcccttggtgttagcccagatctcctgcctaaagactttgcaagctattgcttttctgagatggcaccagtc
tgcgcagtagttggaggagttttggggcaggaaatcgttaaggctctatcccagagagatgccccccacaacaacttcttcttctttgatgggagatcaa
gcaatgggatagttgactgtctgggttccaaatgagaagtgcttcagcgtgcaagaccgaactgaaggaggggctagcaagtgttactgctggctgctat
acaggacaatgaccgcaagaatagtcacacatacatgaagaaaaaaataatcctgattcccacgtacatgtatcttgtttcagtagacagaatttctcat
acagggaaaacagataaattattttgcaacaacattggcattatcctcggctgatccttaccacagttatattgatacccctggtaatgaaaaagataac
ccccgcttctttgtacagtgacaaaataacattggtaacatggttacattttgtatatgcttgca
3' EST: DT390260
[+]ttttttttttttttgaggtttgaaacatcctttttttattacaaaatgtaaaaaaaaaatgcagtaaggtatagaagagagtttacagttgcatttggaa
taaattggcagccaatcaattaacagatgtagcagacggatacaacatctgccttttgtttcacaaccattctgcatgtagctctggtgcaaactgattc
catttgaatctgaatattcaaaaaattgtcagatttagaatttttcatttgtgcttactaaaatcttttggccagagaaacaaaacaaatggattggcta
aagttgattttgcaattcagcccttttaagccaataaattaatttaataaatgctaaatgcttgggcaactttgatctgcgatagggtcagtagcaaaaa
cacaataccatacacctgcaagcatatacaaaatgtaaccatgttaccaatgttattttgtcactgtacaaagaagcggggttatctttttcattaccag
gggtatcaatataactgtggtaaggatcagccgaggataatgccaatgttgttgcaaaataatttatctgttttccctgtatgagaaattctgtctactg
aaacaagatacatgtacgtgggaatcaggattatttttttcttcatgtatgtgtgactattcttgcggtcattgtcctgtatagcagccagcagtaacac
ttgctagcccctccttcagttcggtcttgcacgctgaagcacttctcatttggaacccagacagtcaactatcccattgcttgatctcccatcaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: