XB-CLONE-3260153
Clone Name: IMAGE:7684016
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Gene:
septin2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127290 [+]
GGAAACCGGGAGTTATACTTTATAAAGCTTCAAGATGTCAAAGCAACAGGCTCAGTTCACCAATCCGGAAACACCAGGATATGTCGGGTTTGCCAACCTT
CCAAATCAAGTTCACCGCAAGTCTGTAAGGAAAGGCTTCGAGTTCACTCTAATGGTTGTGGGGGAGTCTGGACTGGGAAAATCAACTCTGATCAACAGCC
TCTTCCTGACAGATCTGTATCCTGAGAGGGTAGTTCCTGGAGCAGCAGATAAAATTGAACGAACAGTGGAAATTGAAGCTTCCACTGTAGAAATAGAAGA
GAGGGGTGTGAAGTTACGTCTCACCGTGGTTGACACTCCTGGCTATGGAGATGCCATGAACTGTGTGGACTGTTTTAAACCAATCATCTCTTATGTGGAC
AACCAGTTTGAGCGTTATCTACATGATGAAAGTGGCTTGAACCGGCGGCACATTGTGGACAATAGAGTCCATTGTTGTTTTTACTTCATCTCTCCTTTTG
GACATGGCCTAAAGCCTCTTGATGTGGAGTTTATGAAGGCTTTGCACAATAAGGTCAACATTGTCCCAGTCATTGCCAAAGCTGATACTTTGACGTTGCG
GGAACGTGAGAGACTAAAGCGAAGGGTATTGGATGAAATAGAAGAACATGGAATAAAAATCTACCAGCTTCCAGATGCAGAGTCAGATGAAGATGAGGAC
TTTAAGGAGCAAACAAGACTCTTAAAAGCAAGCATCCCTTTTACAGTAGTTGGATCCAACCAGCTCATTGAAGCTAAGGGTAAAAAGGTGAGAGGCCGAC
TGTATCCCTGGGGTGTGGTGGAAGTGGAAAACCCAGAACACAATGATTTCCTGAAACTGAGAACCATGCTGATCACACACATGCAGGACCTGCAGGAGGT
AACCCAGGACCTGCACTATGAAAACTTCCGCTCAGAACGTCTTAAGAAAGGTGTTGCCAGCAAGGTAGAAAATGTGGAAGTGACAAAGGACCAGATACTC
CAGGAGAAGGAAGCTGAGCTCCGTCGCATGCAGGAAATGATTGCAAGGATGCAGGCACAAATGCAGATACAAAGTCAAAGTGGTGATGCACAGCACTTGT
AATGGTCTACAGTCAATATATTAGAGCCAAAGCTTTGGGCTAACAAGGATTCACATGTGGCCTGCTGTACTGTACACAAAGTGCTTTATTCTGGTTCATA
CATACTGACATTTTGTGAATTGACATCCAGTTTGTTGCAAATCTTCTATATACATGAGTTACACAGATTTTGGATCTTTGAACTGCCACTTGCTGTTACA
CTGTTTGTTTTGTCTGTTACATGTTCTGCATTTTCTAAACACAGCCTATAAAAATATAATGCAGCTTTATATCCAGATGCCAGGTATGTATGCCGAGCAG
CAGGTATAGGCTGTACTTTAATCTGCGTACCTATTGCGTCTTGCTTACAGAAATATGTCCTCTTAACTTTCTGCACTTCTAGCTTTAACCCCCTACTGCC
CTTTACCTACTGGGGTTCTATTACTCCAGTCTGTTGGAAATATATAAAACCAAAATGTTCTAAGTCCTGCAAGGCAATTAAAGCCCCACAAGGATTTATG
CTACAAATTTTTACATTATACACAGTGCTGTTCTACTTGCCTGGGGGGATACAGGAGCCTTGTGACGTAAAGAATAGCTTCCACTCTTCTATATCCGTAC
TGCTGACATTAGGCATACCCAGATTGAGAGTAAGGTTAACCAAGAAATATATGGCGCTATAGGGAACAATTGTTTATGAATAGTTGTTTACTGACATTGT
AATGTAGTATATCTGGGTTGAACAGTAAGCCTTGTATTTGAGATTCTTGCCTAACTGTTCGGCATTAGTTGTGTATATACCAAAGGGTTTTATCCTTAAC
TAATACAGGCAAGTACGATTCCATAGCTCTAGTATGCACAGAACAATTGTTTTTCATTGTTATTCCTAACACTGAGAGCTCTCATGCTAATTTCTTCAGT
TTCTACTGTTTATAAATATATGTGATGCAGTATCACCAGCTGTCTATGGCGGTTGTTTCTGCTTTGTCAGACAATAAAACAAGTCCTTACTGTAAACAAG
CAGAGAAAATGTGGTGCACCCATATTAGTCTAAGGGAAAGTGAAAATACTGATGTCCTATCAATAGCTTTTATCTTCTATTAAAGCTACTGCACTTTACA
AAAAAATAATACTCATTGTTTTTATGAAAAATTGCATTTTTATTAGGCATCTGCATGGCAACTGTCGGGAAGAAACACATAGAAAAACTGTACTGGGCCA
GATATTATTTGGGATCCCAAAAGGGGGAATCATTTCAGTGTAGTTATAGCTATTTGGAAAACCCCAGAACATGTAATGTTAGAAAATTATAACCAGTTTT
TTACATTTACATCCCCTTTATTAGACCAAACAAAATGAGTGAAAGAAATGTGCTCAAAGTTGATTCATTGTTTGTTTATATGAAGTTCCAGTTATAAACA
GAAGCCACCATATTGTGGATCTGAATAATACCATTAGGAGTGCTGTTCCATTTAGATCTCAGGAGACTGCTTTAGAAGAGGAAAGCAACACTGTGATGAG
AAGGAGTGTTCTTTCTACAAATTAAGATAACTTCCTTAGTTTCTGCTGCTTAAGGAAAACTATGTAGGTCTAATCACAATTGCTAAGGCATCTGCCTACC
TGCTCCTCCAGGTGTAAACTTTTAGGGCATACGTGAGCTGTAGCAATGTATAGGGGCTATATTTGAAAGGTCAAATTATGGCATTAGAGGAAATGGACCA
AAAAAAAATCCAAGCTTCACATGGGAAGGCATGCTCCCCATTTATGTTCAGCAAAACAGGAGTATTATCCAGTGAATCTTGAGCAAATTTGTGTATTGAA
ATCTTCTGCTTGGAGCTTTGTAGTCAAAAAGACCTCAGGAGCACCTATTCAGGGGCACCCAAAGATTGTACAATGCAGCTCATACCAACTGTTTTGTCTA
CTGTAGAGCTTGCTTGTTTGTTACTATATGATCAAACAGTTTCTTGCAGTTTAAGGCCATAGCATGGACTAAGAATAGAGAAAGATTTGTCTTTCAAGAG
TATTATAAATTTTTGACATATGTGCTTTATTTGGAAGTGTATTGAGATTTTGTTGACCTTGTTGACTTGTCATAGGCTGTGTGGGGATACACCATGGACT
CAGTGTGATTATTTTGATTGCCGCAGGTCTCTATGGTAGGGAACAAGGTAAACAATATGCTTGTTATGTAATGAGTTTTAAGCTAGCTATGTACTAAATT
ATTTAATCCCTGCTTGATTTCTGATTGATACTGAATAAGAAGGCTGAAAAAACCCATACAGTCTGTACAGGTCCCCCGTGAACTGATTGTTGGCCCCGGG
ACCAAACAGTTGGATTCATTTTATTTAGCTCCGTTGGATGGCCAAAAGCATTCAAAGATCTGTTTGCTGGGCAACCTTACCAAATGAACAAACCTTTATA
TTAATGGGCAACTAAGGGACATCTTTATTATAGTGTGTAAGCCATCATTATTGGTGATGTTGCCAATTGCAACCAATTAGATCTTATCTTTTGTTTTCTA
ACTTGTAGGCAAATGTTCAAATCTAATAGGTGATCGGTTGCTATGGACAACATCACCGTGATGTTGGCTTACACACTATAGTAATTATGACCCTTAGAGT
TTGCATTAATTTCCAGGTGGTGTAGCTTTTACAACATAAACATAATGCTTTCCTATCAAATAGATACCATCTATGAAATTGTAATAATTCTGCTGTCACT
CTATGTAAAGCACATACTGCCATTCCTCTACATAACATCTTTGGCTTCTTACTGCACTTTATAGCATTTTTTTAAACTCCACAGTAAATTAAAGGAGTAA
CTAATTAGAATGGTCTCCTGTACTTTTATATTTTATATATATGTTAAGATGTGGAGTATTTGAGACTATTGCTTTTTTTTTTTTTTTTTTTTACATTTTG
ATAAGTCTGTATCTTTTGTATCCTACCACCAGGAATATATATATATAACTGTGTGCATTCATAGAGATAAAAATTTAACTTCAATGCAGTGCCTTTTAAA
ATTGTAATTGTTATGCTGTTTTCATTGTAATGGTGCATGTTTCTTGTGTAAGACAGTTGTATAATAAAATTTTCAATTAATCAGTGGGAAAATATGGTGC
AAAGGAATCATGTTTTCCATTTAAATGTACATTTTGATAATAAACTTATAAATTGAGAATGTTGGTCAGAAGCTTTATATATACACACAGAGGGGCACTG
GATACAATAAAACTTTCCTAGGAAAACCTGCCTCTTCAAAGGTTGGTGCTACATGTAAATGTGTGAACAGCCATTGCATAAGGATGACTTACACCTCAGG
CCTGGGTCAAGTCAATGTTCTCTCTATTGCCTCTCCTGCTCCTGCATTACTTCTGCATATTATAACTGGCAGCAACAAGATGTGGTCAAAATGCCTAGAT
TGCCTTGGCCCTTACAATTTATATAATACAATTGTAACTTTCTCTGAAGAATATAGTACAGCTTTTAGTGTTAGAAAAGGTGCTTGTTTACCTTTTGCAG
GACTCTAGGACTCTGCAAAAGAGGCACTTTCCCAATACAATAGTTTACCAAAAATTGTTTTAGTGAATAAAGCTGCAGTCTCTTCTACTGGCTGCCGTTT
TCTGCACTGCGCTTAATCCTAACATTGCGCAAGCTAAATCTTTGAATATCATTTTTGGTAACATAATTTATTTGGAAAAGTGCTTCTTTTGCAAAGTCCT
GCCAGTTTGGAATGACATAGCAAATGTGAACATTCCATTAAAGTACTAATTGGACACCGTTCCAAAGGAATCCTGCAGTACTGTTTAATCCTGTATCACT
CTGTCTGTTGAGCTAGTCAAATAGTATATAAGGCATGTGCAAAATCCAAATACTTAAAATGTTTTGCAGGGTTAGGCAGAATTACAGCTTCTTCTGGTAT
ATAACTGGGGTACTTTGATACTTATGACTGTTGGTGAGCAACTGCTAGCACAAGGAGGGTTACCAATCACTGCTGCATTGTTTTTTCTTGAGTAGCAGCT
GAGCATCTGTTTGCATTCCATATAAATGCATTTGGATACAGCTATGTCTTTTCTATTTATATGTAGTCAGAAATTAATGCTTTGCTGTGTCAAACTTTGA
TATCCAAAGCCTAAACTGCCACCCACCTTTATAGCTGCAGAGCCATTTTCACAGACTTTTCTGAGAGTTCTGCTCAATGTATGGAAATGGCTCAAAACAG
TTTTCCACTGTGCAATGTAAATCTTGGATTTTATATACTGCAAGAATTTATTTTCATTTTATGGGATCTACCACTTTTTGCTTAAAATGTATTTGTATAT
GAAACTTTTAACCTTTGTTATAACAATGAATAAATGGTTTTGTTGGAAAAAAAAAAAAAAA
5' EST: CX902308 [+]gaaaccgggagttatactttataaagcttcaagatgtcaaagcaacaggctcagttcaccaatccggaaacaccaggatatgtcgggtttgccaaccttc
caaatcaagttcaccgcaagtctgtaaggaaaggcttcgagttcactctaatggttgtgggggagtctggactgggaaaatcaactctgatcaacagcct
cttcctgacagatctgtatcctgagagggtagttcctggagcagcagataaaattgaacgaacagtggaaattgaagcttccactgtagaaatagaagag
aggggtgtgaagttacgtctcaccgtggttgacactcctggctatggagatgccatgaactgtgtggactgttttaaaccaatcatctcttatgtggaca
accagtttgagcgttatctacatgatgaaagtggcttgaaccggcggcacattgtggacaatagagtccattgttgtttttacttcatctctccttttgg
acatggcctaaagcctcttgatgtggagtttatgaaggctttgcacaataaggtcaacattgtcccagtcattgccaaagctgatactttgacgttgcgg
gaacgtgagagactaaagcgaagggtattggatgaaatagaagaacatggaataaaaatctaccagcttccagatgcagagtcagatgaagatgaggact
ttaaggagcanacaagactcttaaaagcaagcatcccttttacagtagttggatccaaccagctcattgaagctaagggtaaaaaggtgagaggccgact
gtatccctggggt
3' EST: CX902307 [+]tttttttttttttttccaacaaaaccatttattcattgttataacaaaggttaaaagtttcatatacaaatacattttaagcaaaaagtggtagatccca
taaaatgaaaataaattcttgcagtatataaaatccaagatttacattgcacagtggaaaactgttttgagccatttccatacattgagcagaactctca
gaaaagtctgtgaaaatggctctgcagctataaaggtgggtggcagtttaggctttggatatcaaagtttgacacagcaaagcattaatttctgactaca
tataaatagaaaagacatagctgtatccaaatgcatttatatggaatgcaaacagatgctcagctgctactcaagaaaaaacaatgcagcagtgattggt
aaccctccttgtgctagcagttgctcaccaacagtcataagtatcaaagtaccccagttatataccagaagaagctgtaattctgcctaaccctgcaaaa
cattttaagtatttggattttgcacatgccttatatactatttgactagctcaacagacagagtgatacaggattaaacagtactgcaggattcctttgg
aacggtgtccaattagtactttaatggaatgttcacatttgctatgtcattccaaactggcaggactttgcaaaagaagcacttttccaaataaattatg
ttaccaaaaatgatattcaaagatttagcttgcgcaaatgtaggattaagcgcagtgcagaaacggcagccagt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: