XB-CLONE-3297599
Clone Name: IMAGE:7662743
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Gene:
kat14
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136099
[+]
GTGCTGTCTGTGGGTACGGTGGTTGGCGTGCATGTGAAGCAGAGACTGGACAGAGAGAGACTGCGTGACGGAGTAATTCAAGACTTGCAAAGGCAGAGTC
GGAAGCAGGAGAACCTCCGCCTACTTCAGGAGCAAATTCATCTAACCAAACAGCTTGAGGCAGAAAGAGATAAAGTTCTTCTCACTGAGGGATCCTCCAA
GGCTTAGATATCAATTCTGCCAAGAGTTTTTGTGCTTAGGAATTGATGCAGCACAGTGACAGAACTTTCAGAATGACTTGGGTTTTTTCTCTGCATTTTT
TGGACAGTTGAAGCTGGCTCTGAGAAATGCCAAAAGGCCTTAAGGTTTCTAAAGGAAACAAAATATACACACTTTTGAAACAAACTAAAGAGATTTTGTG
ACTGGATTTTGGGAAATGGATATCTCTCTTACCAGTGTTGATGATGAAGCGACAAGAACATCTGCATCAGAGGGACTGGAGGAAGGCGAAGTGGAAGGAG
AGACCCTTCTGCTAGTTGAGTCTGAGGATCAGGCATCTGTTGACTTGTCCCACGATCAGAGTGGGGATTCCTTAAACAGTGATGTTGGCGATGATGTGGA
CCCACCATGGATGGAGGAAATGCCTTTTTATTGTGACAAATGTCAAAAGTGGATTCCAGCAAGTCAGCTGAAAGGGGATCCTCCGAGTTATCTTAAAGGA
GATAACTACTTCAAATTCATATGCTTTGCTTGTTCTGAAGATGGCAAAGAGCAATTTGAACGATTAAGACTAACGTGGCAGCAGGTGGTTATGCTTGCAA
TGTACAATCTCTCCCTGGAGGAAACTGGGCGACAAGGTTATTTCAGATGGAAAGAAGACATCTGTTCTTTTATTGAGAAGCATTGGAACTTCTTGCTTGG
AAACCGAAAGAAGACCTCCACCTGGTGGAGCACAGTCGCAGGATGCCTTTCGGTAGGAAGTCCTTTGTTCTTTAGATCTGGGGCTCAGGAATTTGGTGAA
CCGGGATGGTGGAAACTGGTGCACAACAAGCCGCCCATCATGAGACCTGAGGGAGAAAAGTATTCTATTGCTTTACAGAAAACAAAGGTCCCAGTGAAGA
CTTCCCTTGATCCGGTTATCACAGTAGAAGGTCTCAGGAAGAGAGCGAGCCGAAATCCAGTTGAGTCAGCAATGGAGCTGAAGGAGAAAAGGTCACGCAC
TCAGGAAGCCAAAGAGATAAGAAGAGCACAAAAAGAGGCTTCTGGCTTTGTGGATCGTAGTACATCCTCCACACCAGTGAAGTTCTCTAGTGGAAGAAGC
CGTCGTCCTGATATTGTGCTAGAGAAGGGTGAAGTGATAGATTTCTCATCCATGAGTTCATCTGACCGAACACCTATGACTAGTCCTTCCCCTTCTCCAT
CCTTAGATTTCTCTGCTCCAGGGACACCAGCTTCTCACTCAGCAACTCCCAGTCTTCTTTCTGAGGCTGATCTAATCCCTGATGTTATGCCGCCACAAGC
ACTGTTTCATGATGATGAGGAGCTGGAAGCAGACAGTGTAATTGATCCTGGGATGGAATACATACCTCCTACCAGTGCATCACTAGCTGGTTCCTCGACA
CCCATATGCCACAAAAAGGTTAAAAACCCCCTACAGATTAAACAAGAGATAGAAAGTGAAGAGGAGAGATCTGAGAGGATTGAAGAAGAATTACCTGAAG
ATTCCTGGCGGGAAAGAAGGCGCAAGAAGAAAGGGCCCCTGCCCACAGAAAGTGACAAAAACAAAGGCACACAGTACAATGCGATAAGCCTTTATGAGGA
AAAACTTCTTTTAAAACGCCTAGAGACCTGCCCAAATGCAGTGGCAATAACTCATGAGGCCAGGAGACTCAGGCGCAAGCTACTTGTCCGTCAGGCCAAG
CGTGAGAGAGGGCTACCCCTCTTTGATTTAGATCAAGCAGTGAATGCTGCCCTTCAGCTGGTAGGCGGAGTGTATGGAGCGAGAGAAGGGTCTGTCTCAA
GACCAGCACCCTCAGGCCCAGAGTATCAGGCAACAGCCAAAGACTACAGAATCTTGGATAGGTACCAGACTGCGCTGCCAGTCACAAAGAGACATCGGCA
TGCAGCAACTCGCTTTGTGTACAGATTGGGGGGCTGTGAAGACAAGGATTCAGACCTCAGCATCGTCAGCCCCTATACTGCCAGAGTCTTGAAGCCCTAC
ATAAGGCGCGACTATGAGACAAAGACGCTGAAGCAACAGCTCCTGGCGGAGATTAAAGCCTTTCCACACAGAAATGAGCCTGACTGGCAACCGGAGCCAG
AGTCTCCTATCGATTACTGTTATGTCCGTCCAAGCCATATACCCACCATTAACTCAATATGCCAAGAATTCTTTTGGCCTGGCATTGACCTCTCTGAATG
TTTGCAGTATCCAGATTTCAGCGTTGTAGCGCTGTACAAAAAGGTTGTCGTTGCTTTTGGCTTCATGGTTCCAGATGTGAAATATAATGAAGCCTACATC
TCATTCTTGTTTGTGCATCCTGAATGGAGGAGAGCCGGCATCGCCACATTTATGATCTACCACCTTATACAGACTTGTATGGGGAAGGATGTCACCCTGC
ATGTTTCTGCCAACTCATCAGCGATGTTGCTCTACCAGAAGTTCGGATTCAAGACGCAAGAGTACATTTTGGACTTCTATGACAAATATTACCCGTTGGA
CAGCAAGGAATGCAAGCACGCCTTTTTCCTTAGGCTGCGGAGGTGATGGAGATACATTAACCTAAATGCCTTGTGGGATATTTGGCAGTTTATTTTAGGG
CAGAAAAGCCAATTAGTCTTTTGTAAGCCGCGCCTCACACGAAATAAAAACAATGTCAAAAGAATCATCTGTGTTTGTAAGAAAGGCTGCGACATGCATG
GAGTAAATGGACCCTGTAGGAGCTAACAAATATTAAAGGGGAACCGCTATTATACCACCTATCGCTGTAATCTGTTCCTTCAAAAAGTATGAATAAATAC
CATTTTATATGCTGAAATCCAGCTGCAAAAAAAAAAAAAAAA
5' EST: CX845317
[+]gtctgtgggtacggtggttggcgtgcatgtgaagcagagactggacagagagagactgcgtgacggagtaattcaagacttgcaaaggcagagtcggaag
caggagaacctccgcctacttcaggagcaaattcatctaaccaaacagcttgaggcagaaagagataaagttcttctcactgagggatcctccaaggctt
agatatcaattctgccaagagtttttgtgcttaggaattgatgcagcacagtgacagaactttcagaatgacttgggttttttctctgcattttttggac
agttgaagctggctctgagaaatgccaaaaggccttaaggtttctaaaggaaacaaaatatacacacttttgaaacaaactaaagagattttgtgactgg
attttgggaaatggatatctctcttaccagtgttgatgatgaagcgacaagaacatctgcatcagagggactggaggaaggcgaagtggaaggagagacc
cttctgctagttgagtctgaggatcaggcatctgttgacttgtcccacgatcagagtggggattccttaaacagtgatgttggcgatgatgtggacccac
catggatggaggaaatgcctttttattgtgacaaatgtcaaaagtggattccagcaagtcagctgaaaggggatcctccgagttatcttaaaggagataa
ctacttcaaattcatatgctttgcttgttctgaagatggcaaagagcaatttgaacgattaagactaacgtggcagcaggtggttatgcttgcaatgtac
aatctctccctggaggaactgggcgacaaggtatt
3' EST: CX845316
[+]ttttttttttttttttgcagctggatttcagcatataaaatggtatttattcatactttttgaaggaacagattacagcgataggtggtataatagcggt
tcccctttaatatttgttagctcctacagggtccatttactccatgcatgtcgcagcctttcttacaaacacagatgattcttttgacattgtttttatt
tcgtgtgaggcgcggcttacaaaagactaattggcttttctgccctaaaataaactgccaaatatcccacaaggcatttaggttaatgtatctccatcac
ctccgcagcctaaggaaaaaggcgtgcttgcattccttgctgtccaacgggtaatatttgtcatagaagtccaaaatgtactcttgcgtcttgaatccga
acttctggtagagcaacatcgctgatgagttggcagaaacatgcagggtgacatccttccccatacaagtctgtataaggtggtagatcataaatgtggc
gatgccggctctcctccattcaggatgcacaaacaagaatgagatgtaggcttcattatatttcacatctggaaccatgaagccaaaagcaacgacaacc
tttttgtacagcgctacaacgctgaaatctggatactgcaaacattcagagaggtcaatgccaggccaaaagaattcttggcatattgagttaatggtgg
gtatatggcttggacggacataacagtaatcgataggagactctg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: