XB-CLONE-3299085
Clone Name: IMAGE:7661653
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Gene:
phc2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC153340 [+]
GCTGAATGTTGGTTCCACCGGGACTCGGTGCCCGCCGGTGCCACCACCATGTGATCCGTCTTCCCCCCGAGACTTCTCTGTCAGCCAATCAGCATGGAGA
CTGAGCAGCAAGCCACGCCCCCGCCGCCCTCCAGCAGCTCCAGCAGCCGCCCTCCTCCTCCTCCTCCTCCAGCTTCCTCCCTGCCCGGGTACAGCGCCAC
CCCAGAGCGGCAAACTGTGCAGGTGATCCAGCAGCACCGGCCCCCCAGCACCGCGGCCCAGTACCTGCAGCAGATGTATGCGGCCCAGCAACAGCACCTC
CTGCTCCACAGCGCCGCCCTGCAGCACCAACACATACAGAGCCTGCAGCAGGTGGGTCTGGCTACCGGCCGGCCGGGAAGTGCCCCCGCAGCGACTGTCC
CCCAGCAGAGCTCCCCCCACTCCCCAGGGATTAACTCCCCGCAGAGCATCAGCCGCCCACAGAGCTCCACCCCTGCCCCGCCCTCAGCTATTACCCAGCA
GGCCGTGCTTCTGGGCAGCGCCACCTCCCCTGCTTTATCTGCCAGTCAGGCCCAGATGTATCTCCGAGCCCAGATGCTCATTTTCACTCCCACAGCGACA
GTGACCAGTGTGCAGTCTGAGGTTAGTGCCGGGGCCCCGCCTCCTGCCGGCAGCACTCAGGTTCCCAGTGTGGCGGTGCGAGCGCAGCACAGCGGCCTGA
GCCCCCAGTCCCACTTGCCCAGTTTGGCTAGAAAGCCGGCCAGTAGCACCCTGAGCGCCCAGCCAGTGGCCAACGCCGCCCAAACCAAAGCCACCGGGCA
CCTTCTCCTTCCCAGCATTCCCGGGGCCCACCTTGAGCCCCCCGACAGGAAGGGCGAGAGTCGGGCAGCTGATGGCCGAGGGGCAACTTCATGCCGGACT
GTGGCCCCAGTCACGGCCCATTCCATTATTGCTCCAGCTCACACCCACCCGCTGATCCAGCAGAACCACAAGCAGATCCAACAGCACCAGTTTGTCATCC
AGCCCCGCGCGGCCCTGCCCCTCCCCCAGCTGGGGGCCCCCCTGCAGCCCTTTACCCATCAGCCCCAGCTGTGCCAGAGCCTCACCTCCCCCCCCGTCAC
ATCCCAGGCACAGCTGGGGCCCCCCTCGACTTGTGCCCCCCAGCTCCACTCAGCTCCCGGGGGCCCCCCCAAGATCAGCCCCGAGGGGCAGGAACGGAGA
CTCCTCCCACACAGCTCCACCGTCATCCTGCAGCTCCACAGCCCCCCCGGAACTACACCGACACCCCAGGACGTCTCCCACAAACGCACCTTTACCCCCC
TGGGAAATAGCCCCTGCCTGTTACACCTACACCCACCAACTGCCCCCCTGGCACAGGAGTCGGCACCCGGGGGGGCATCCGTCACTGAGACGTCAGACCT
CTGCCAATGCCAACCTGATGACCCCAGACCCCCGGCCCTGCCGGACCCCACAATTCCCTCTGCGTTGGACATGACCTCAGGGAACGGCTCCGCCCCCGTG
CCCACGGTCGCCACTGGCAACAGCGCCCAGAATGGTGAGAACAAACCCCCCCAAGCTGTCGTCAGACCCCAAATCCTCACTCACGTCATCGAAGGATTCG
TAATCCAGGAGGGGGCGCAACCCTTCCCGTCTCACAGGAGCAGAGCTGCCCCAGAGGTCGGTCGCTCCTCTCTGCTCATTGGTGCCCACGAGAGGTACCC
GCAGGGTCTGTTGGCAGAGAAGGTTCCTCAGCAGGACAGTAACACCACAACCACCACTACTGACTCCGAGATGGAGGAGACCTTTGTACCAGGCTTTCCA
GATTCTAAAGGAGATGGGGATCCCCCTAAACTGAAGTGTGAGCTCTGCGGTCGAGTGGACTTTGAGTACAAATTTAAGCGCTCCAAGCGGTTCTGCTCTA
TGGCATGCGCAAAAAGGTACAACGTGGGCTGCACCAAGCGCGTGGGGCTCTTCCACCCAGACCGCAGCAAGTTACAGAAACCTACGGGGGCCAAACACGC
CCGCAGGAGAGCGCAGAAGGGCCCCCTGCAGAGTGTCAGTACTGACCCCAAGAAACAGGTACCTGCATCTGCCCCCCCTATTGCCCCCGGGCCTGTCCCC
GCCCCCTCGGCCCTAAAGCACAGTAACAGCCAAGAAGACTCCAGCCGCTGCTCGGACAATTCCAGCTACGAGGAGCCCCTGTCCCCCCTCTCCGCAAGCT
CCTCCCTCTCGCGGCCCCGACCGGAGCGCAACCTGGAAGCTCCGCCCCCCCTCCACAGCAGGGACCACATCGCGTTGAACCAGGACTTCCTGCCCAGCAA
CCCGACCAAGTGGAACGTGGAGGACGTGTACGAGTTCATCCGCTCCCTCCCAGGGTGCCAGGACATCTCGGAAGAGTTCCGTGCCCAGGAAATTGATGGG
CAGGCCCTGCTCCTGCTGAAGGAGGATCATCTCATGAGCGCCATGAACATTAAGTTAGGCCCCGCCCTAAAGCTCTATGCTAGGATCAGCATGCTGAAGG
ACTCCTAGGGGGCGGCGCCCTTCCTTGGGCCAATGGAAATGGGCCAGTCGGGCACCAAGCATTAGCAATAGACAGACGCTGGCAGATCTGGGACAGGCTC
CACCCACAGATGCAATACTTGTTGTGCCCCTTGGCAGGACCCGCCCACCTTCTCCTGGGACTTCTACCTTGGGATCCTCAGGGGCGGGACCAAGCCAATA
GGAACTCTGGGATTCGCTCCTCCCACCAAACCTCTCACTTTGTATTTTCAATGAAAATGTCTTTATTTTGAGAAAAGCTCTTTTTGCAGAATTCCTGTCA
CATTTCTATGTGGTTCTGATCCCGGCCCACTTGCTCCAAAATTCAGTTTTTTTAACAGTTTGCAGAATAAACGGAATAAAACTTAAAAAAAAAAAAAAA
5' EST: CX842947 [+]ctgaatgttggttccaccgggactcggtgcccgccggtgccaccaccatgtgatccgtcttccccccgagacttctctgtcagccaatcagcatggagac
tgagcagcaagccacgcccccgccgccctccagcagctccagcagccgccctcctcctcctcctcctccagcttcctccctgcccgggtacagcgccacc
ccagagcggcaaactgtgcaggtgatccagcagcaccggccccccagcaccgcggcccagtacctgcagcagatgtatgcggcccagcaacagcacctcc
tgctccacagcgccgccctgcagcaccaacacatacagagcctgcagcaggtgggtctggctaccggccggccgggaagtgcccccgcagcgactgtccc
ccagcagagctccccccactccccagggattaactccccgcagagcatcagccgcccacagagctccacccctgccccgccctcagctattacccagcag
gccgtgcttctgggcagcgccacctcccctgctttatctgccagtcaggcccagatgtatctccgagcccagatgctcattttcactcccacagcgacag
tgaccagtgtgcagtctgaggttagtgccggggccccgcctcctgccggcagcactcaggttcccagtgtggcggtgcgagcgcagcacagcggcctgag
cccccagtcccacttgcccagtttggctagaaagccggccagtagcaccctgagcgcccagccagtggccaacgccgcccaaaccaaagccaccgggcac
cttctccttcccagcattcccggggcccaccttgagccccccgacaggaagggcgagagtcgggcagctgatggccgaggggc
3' EST: CX842946 [+]tttttttttttttttaagttttattccgtttattctgcaaactgttaaaaaaactgaattttggagcaagtgggccgggatcagaaccacatagaaatgt
gacaggaattctgcaaaaagagcttttctcaaaataaagacattttcattgaaaatacaaagtgagaggtttggtgggaggagcgaatcccagagttcct
attggcttggtcccgcccctgaggatcccaaggtagaagtcccaggagaaggtgggcgggtcctgccaaggggcacaacaagtattgcatctgtgggtgg
agcctgtcccagatctgccagcgtctgtctattgctaatgcttggtgcccgactggcccatttccattggcccaaggaagggcgccgccccctaggagtc
cttcagcatgctgatcctagcatagagctttagggcggggcctaacttaatgttcatggcgctcatgagatgatcctccttcagcaggagcagggcctgc
ccatcaatttcctgggcacggaactcttccgagatgtcctggcaccctgggagggagcggatgaactcgtacacgtcctccacgttccacttggtcgggt
tgctgggcaggaagtcctggttcaacgcgatgtggtccctgctgtggagggggggcggagcttccaggttgcgctccggtcggggccgcgagagggagga
gcttgcggagaggggggacaggggctcctcgtagctggaattgtccgagcagcggctggagtcttcttggctgttactgtgctttagggccgagggggcg
gggacaggcccgggggcaatagg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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