XB-CLONE-3538011
Clone Name: IMAGE:7719938
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Gene:
siglecl2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983773 [+]
cccttctgaacatcactattccaggggggacaggagagagcagcagtctaattgtgagagaaggcgactccaaggaaatctactgtacagttgacagtaa
cccaacggccaacattagctggactagaggaaacagcccggttgggaataccaagcagaatgggcagaatctgactctttcactacagaatgtaacagag
aatgacaccgctgtatatacatgtacggcacagaaccaaagggggagcaacagccgctctgtcagtgtctctgtggaatatcccccccgacagccaacga
tctgtgtagataagccggaaggttgcctttctttagtaaatgactctgttccagaaggatcgacactgtccttaaactgcagagcaaaaagtaaccctga
ggcaattatttcctggattcagaatgatccactcaacacattttctgaaaacagtattctaaaggtccaggttacaaatgatattgtatatacatgccaa
gctacaaatagatacggactatcaaatagttccatccttattattgtcacgcctgtacaaaagtctcagacagttttcctcattgtgggactttgtattt
tatttttcacacttgcggtgatagcggcggtgcttgtattcatatggatgagaaagaagaagaacgtacaggaggacactaaagagatgcctaagagtga
tggtgatgtgatttacaatgacgtagagaatatacaaagcatcaacaatgaatcctacatggacacgg
3' EST: CX983772 [+]cttggctggttgtccaacagtgatttgctcttattgtataactaaaaaatactggcaaaagtagtttttctcagaacaggtatgggacctgttatccaga
caacttgggatctggggttttccagatatcactattaaaaaaataatatatacattaaataaacccaataggattgttttgcctccaataaggattaatt
atatcttagtttagatcaagtaaatgttgtctgactggcttattattacagcggaaaagcaaataaatgtaaaaaaatatcattattgaaatactaataa
ttacaatttttgtgggattcccaaaaataaattaaatataaagtctggttggaaacctatacactttacctgtgcaagcttacacagtctagaacagtga
tccccaaccaatggctcggggcaacatgttgttggatgttgctctcagtgcccccaaaccagggagttatttttgaattcctgacttgggggcaagtttt
ggttgaataaaaacaagatttcctaccaaataaagccccctgtaagctgatagggtgcatagaggctccctaatagccaatcttagcccttatttggctc
ctccatgaacttttatggtgcttgtgttgctctccaagtctttttacatttcactgtggctcacgagtaagaaaggttggggatccctggtctagaagaa
ccccgtggtaccccaagaatcagacataatctgcagcgtctgtatctacattgtcccaatgagtctctatagtacaacggtccttctttatttacatttg
tggatttcttcagtatatcttaatggttaatagcatttgacatcagaatataacatctcctctgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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