XB-CLONE-3543314
Clone Name: IMAGE:7717908
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Gene:
selenop1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980155 [+]
tttttttttagtttgtgtagaattttattgtttatagaaatacactctcacttggcttcccatactgcattaatgctacttagcaatgccatataggttt
attcaggttatgtaggagtttgtttccctagaaaccgtcattgattatagcatttactaacaatgcagcccatcaccaaagaacttccaaacccttctct
aaaagcactacaaaaagttcatacatacaaagaataagacagtaatatcagcaataaaattcattacaaatgcaaataactaaagcaaaacacctgcgat
ctttggcaggtcctggtatgcgatggcttcacaatggcactctattgacaactcccagtaacctccaaaaacgctggaggttgctggcagttacagtaag
agttcccctattaagtcaccaaagaacataatattcagtctacaatattctaatgctatcttgtgttctcaatgatagaaattattcttttgcataagca
aaatcttctcacgccattcaccagtttctgtttgccagactcagacagaagcccctattttgtagtttatgcttctgccatcattaacgcaagcaacaaa
tcaagtgatgaacaggagaggcactgtggtggtgtggaggtgcgctgcattcacattagcctaggatgttataaaggctcccttggtcaggcctgcttta
actatatcatgcccaggtttcagcaactcattgctcagcaatcaattgcttcactcctgggcagttaaacagttaaataatgatgttgggagattgtgca
ttacactgatggattagcagcccattaacagctattttct
3' EST: CX980154 [+]ttggtgcaacatctcgaactgccgtactcactcttacatttcccttacgttgaagaagccataagaattgcttattgtgaagataaatgtggggaatgtg
aacacaagatactagatgctgatgtttgcaagaaaccagaggagcagcaagagcaagacaaaccagttgaagagaaggtggagagacccagacatcacag
gaatcaccatagacaccaccgccctaagcacagtgggcacagacaccgacatcatcacagtgaagatggccaagtggccgaacttgatgtccttaggtct
agcgctcaagctaacaatagggctggcagtcaaaatgggcagggtcaaagtgttgtgcaagttgtaccacagagtgaggttttatttgtgccacaaaggg
aggcagacattccagtattagctcgacagccttgaaaaaaggctaaaagctgaaagaaacagtatttatgagactggagagaggagggaggcaaggcatt
taacagctgatgctgacactgacgacagctgtcatttgaagtaacagaaaatgaagtggcttgacactgacaggaggctttgccatcttcctgcagttga
caagagctgctgtcggacagcctccctgaatcctgacagtgacgtctgtctgccgctgcatgacattcagaaagcacaggcctgccagagacaaagctag
agtcagagcccaatgccccctgagcctgacctcaggaggcagaaaactgacaatgaaagggaactctgacgattcctaatgtaaactgttaggaatttag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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