XB-CLONE-3549752
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Clone Name: IMAGE:7715849
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Gene:
rpl30
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975713 
[+]
ttccttgatcttatattaaaataataatacatttataacaatgtatacaagttattttgtttttacttggcttcaagaagccattgctacttacagtagg
aacatactataaatctattggtttaagtctacaaataaaaggtatttccctttgtttatggatatgattggattctagtctccagccaaccaacctagaa
gcattgcttctgttctgcacacttttcatatgtctgaataaactcttgatatctgctggagcacagcctacagggtaacaccaaagggcagatttattga
gtttgtgaaagaaagtgggaaaataaataaaaaaaagttaatagagacaattctcaccagcttttacttgccaagttttttctggcaaattctggcaact
gggggatttttttctttaataaattccgactattcatggttattaagattattctcaagtatcatgaaaaaaatgcaaacttgaatttttataaatcggc
ctttaatataaagagcttaaatgtgtgatgaatttctaaaaaataccattatcacccactatgcccatccttggcccctttcacattggcctacagatct
gattattatcatgtgtggcactaataccaaagcagctcctatggtatagctgatacttatacaaattaatacctgaagggatgtgtccagaaatttatag
ttacaaaaatataaatatattgagaaacctgttccagccagaaacaatatggctaccaagacaaaatggtctccctttctttggttttctttgtaagga
3' EST: CX975712 [+]ttactgacagcagaaactcaaggacaaggaggtgaattaatttttggaaagatctgcctggaaacgaaattgatgaacaaaattcaatgtaaaaggctga
aaagttatgtcgagcaaacttctccagttgcagccatcatgttcactacccaaaagaatataacaatctgtgcaaatcctgaacaaccttgggtgaaaca
ggctgtgcaatgtctcaaaactaagaagccaagaagaccaaacccaaagaaaaggaccaagaaaaagaagaatggaaaagcaaagagcaagagcactcct
taagaaagatctttaagttcctcagattgtttcagatttgtttcacaccataattaagccaataactaagctgtgattctctctatcattaaataagtcc
agtattaccgcatatatcatgtagtattgtgaattttgcatccagttaaacacacagggctagattggttagtaggaagaaagaaaagaagtgatctcta
aaagatatctgatctgtggccaacgagtcccctttgggcagatattcctatctaaacatttcctgcaacatattctatagcattcctgcatctgatgtag
aggatattagtacattattacagagttattgtatgttgttagacaactattcttttatccttacaaagaaaaccaaagaaagggagaccattttgtcttg
gtagccatattgtttctggctggaacaggtttctcaatatatttatatttttgtaactataaatttctggacacatcccttcangtattaatttgtataa
gtatcagctataccataggagctgctttggtattagtgccacacatgataataatcagatctgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
