XB-CLONE-3554465
Clone Name: IMAGE:7714055
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Gene:
selenop1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972463 [+]
cttgccctggctctttgccttctcccttggggaggggcagagagccagggccatcgaaagcggtgtaaagaaccgccagaatggagcatcggagaccaga
atccaatgatgcaatctgctgggaaagtgacggtggtggcccttcttcaggctagctgatctctgtgcctgctgcaggcttccaggttggaggatctgcg
gttgaaactggagaaggagaaattggtgggtatttcctacgttgtggttaatcaccaagggagacaatctcgggcaaagtatgatctgctgaagtctaag
gtctccgagcacattcctgtgtatcagcaagaggaataccagcctgatgtctggagcctactgaagggagacaaggatgatttcttcgtttatgacaggt
gcggacgtttggtgcaacatctcgaactgccgtactcactcttacatttcccttacgttgaagaagccataagaattgcttattgtgaagataaatgtgg
ggaatgtgaacacaagatactagatgctgatgtttgcaagaaaccagaggagcagcaagagcaagacaaaccagttgaagagaaggtggagagacccaga
catcacaggaatcaccatagacaccaccgccctaagcacagtgggcacagacaccgacatcatcacagtgaagatggccaagtggccgaacttgatgtcc
ttaggtctagcgctcaagctaacaatagggctggcagtcaaaatgggcagggtcaaagtgttgtgcaagttgtaccacagagtgaggttttatttgt
3' EST: CX972462 [+]tggcttcccatactgcattaatgctacttagcaatgccatataggtttattcaggttatgtaggagtttgtttccctagaaaccgtcattgattatagca
tttactaacaatgcagcccatcaccaaagaacttccaaacccttctctaaaagcactacaaaaagttcatacatacaaagaataagacagtaatatcagc
aataaaattcattacaaatgcaaataactaaagcaaaacacctgcgatctttggcaggtcctggtatgcgatggcttcacaatggcactccattgacaac
tcccagtaacctccaaaaacgctggaggttgctggcagttacagtaagagttcccttattaagtcaccaaagaacataatattcagtctacaatattcta
atgctatcttgtgttctcaatgatagaaattattcttttgcataagcaaaatcttctcacgccattcaccagtttctgtttgccagactcagacagaagc
ccctattttgtagtttatgcttctgccatcattaacgcaagcaacaaatcaagtgatgaacaggagaggcactgtggtggtgtggaggtgcgctgcattc
acattagcctaggatgttataaaggctcccttggtcaggcctgctttaactatatcatgcccaggtttcagcaactcattgctcagcaatcaattgcttc
actcctgggcagttaaacagttaaataatgatgttgggagattgtgcattacactgatggattagcagcccattaacagctattttctgccac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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