XB-CLONE-3560516
Clone Name: IMAGE:7711461
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Gene:
tubb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968250 [+]
cgaacatctattacgcctgaatcatgagggagattgtacacatccaggccggccagtgtggcaaccagatcggcgctaagttttgggaggtgatcagtga
cgaacatggtattgaccccactggcacctatcatggagacagtgatctgcagcttgacagaatcagcgtctattacaatgaggccacaggtggtaaatat
gtccctcgagccatccttgttgatctggaaccaggaactatggactctgtgcgctcaggaccctttggacagatattcaggcctgacaactttgtctttg
gtcaaagtggtgctggcaacaactgggcaaaaggccattacacagaaggtgctgaattggtagactctgtactggatgttgtgagaaaagaggcagagag
ctgtgactgcctacagggcttccagctcactcactctctgggtggtggaacagggtctggtatgggcactctcttgattagtaagatccgagaagaatac
cctgaccgcatcatgaatacctttagtgttgtcccatctccaaaggtgtctgatacagtggtagaaccatacaatgcaactctttctgttcatcagctag
ttgaaaacacagatgaaacctactgtattgataatgaagcactctatgacatctgcttccgtaccctcaagcttactacaccaacatatggagaccttaa
tcatcttgttagtgctacaatgagtggagtcactacctgcctccgtttccctggccagttaaatgctgatcttcgcaaattggctgtcaacatggtgccc
ttcccacgtctacactttttcatg
3' EST: CX968249 [+]caaaaaagggttttatttttttccgtcacaggcttgtcatcttttcaatatgaaaaagcaacagtaaaataaaaataatggtatgggaacaagagaagaa
aaagggaagtaatgtaagaaaatctgaagagtaaagttaagagaagaagaagaaataatccctatatcactgtgtagagttctttagcattcttctgcac
ttttttctgttacttgttaagtaaaatgcagagcacttctctacatacaactgcaggcaatgaaccatgccagttacccaatggatagtgggcctatatg
attaatgtagcttataattaaccaaccaatactgtttacagtaaagggaattaagtgcatttgttaaacatcagtttcatttcatattttctaattggaa
gtattaaggtatttcaaatacttgggatttacataaatgtgagcaaaaccgaatcatatctattgctctagttgacagaagttgcagttacgtgtatttt
aacacttactaggtcatttccatagtaacataagaaaatgtgaatgtccaccaaacacttgaatgtgatctgtaaaagcacagaaagaagcaaaatctgc
aagggttttgtaaagtcccaagggactaaattagaggtttgctttgtaacccaaatgtagctgcagaaaccagcacccagccacaaatttgatgatgtta
ttaggccaaggaaagatggaattcacaatgggctgttaaaactctcttaaatccaaaacccaaatccagaggcaaattactgcatactgaaaccagagac
ctactgtaaaacctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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