XB-CLONE-3560871
Clone Name: IMAGE:7711617
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Gene:
ythdc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX968003
[+]
acacgcttgcttgactaaaatttccattctgaacgtccccccggtcctgggtcgggtggctgtccccacgggatttgcttcgccacggcaggagagcggg
gcgcccggcggggcgggggggacagcgtgacatccaacagcgttggttttagtagtaggcgccgccggtgtcggaggaagaggaggaaaggcggaacgtt
gcggaatagcacagatagaagcatggaggcggagcgaggggaacagagtaaagaaggtgaagtgaatgttttggatgatattttaacggaagcgcccgat
caagatgatgaattatacaaccctgaaagtgaacaagatgtgaatgagaaaaaaggttccaaacgaaaaagtgaaagaattgaaatgactgaaaataaga
gacaaaagcagtcttcacatatgagcagacaacctcctcaaagagtagcatctggggctaacaagaaggtaatcagcaccaaagggaaaccagttacaga
atacaagtgtgatgactttcacaggcaagacaggaacagaaggtcagatggggacagaagaatccgctcctcaagtggcaccgcaagagacaattataaa
ggcccacaagataaaatcagcatcaggaagagagaatctgacaggcgacctaaatccagtacaccggatatggcttctgaganaataagacatgaagtaa
gcagacgtcaaagcagatccagccattcttcaaatgaaggcaactctgatgattatggctctgaaagggaaactggcagcagtgaatcatcagatgaa
3' EST: CX968002
[+]gcgagaggctttttttttataatcacagtggcttttattataaaactgcacaaaataacgcataatggtacaagactacacaaaataagaggtgcatttg
tagcagaggaaatatcgtaactgctagcatatggcttaaaaaaaccactgcagtaacaaattaaattacattgttatcagccaggtacaaaatgatagca
ggagaaaacatggtcaaaatattttttctagcacataggttgcttgcattctccacaagtctgcaactgagaaaaagtgaagaggtacaaatttagacgc
aagtgtttaaaaaaaatgtaggcaacaggctttccagtattgctttcatcattgccaaatagtacaaggataaaagggcagaagggtgtctacaatatgt
tcatcagcccataacttcagaatttgcatacttcatagcagcattatattcacagaatcctcactaactacaaaaggaacagggaaatctgtgaaggcag
ttaacaaaccacttcatcagtggcctttgaaaacagggtatatgcaatacggcccatggagtaacgaacaaaaacgctggtatgaaataccgctaagatg
aacaattaaccaaagacagtttatttctttgcactagtataggctttttaacttgcatctttttttggccattttaataaatctccatttcatacacacg
tgcgcaacagaggttcaaatgatataaaatgagcagtattctcttttgcctaagcattcgacaaactgaacagaaatgtcactattttgtaaacacgcgt
aaggagaaaatatgaaggtaaatttcagagaggacataaccagtgttggccaggaattnatctcctgtatctgcccctat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: