XB-CLONE-3561101
Clone Name: IMAGE:7711212
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Gene:
sdcbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967846
[+]
caggtctgtaaaaatgtctctgtatccctcactggaggacctgaaggttgacaaagtcatccaggcacagacaactttttctgcaaacccagtcaatcca
gcaatattgcctgctgcttcagcatcagctcctcaatatggagggatgtaccctaagctttatccagaactggaacagtacatgggcttaagtctcagtg
aagatgaaattcataaaaatatgtctttggtaccttctggtggggtacgtaaccattcatctgcaactgtccaatcagattacagcatttcattagataa
agaaatatattcagagcttattttatagcacattcttcgctctgaggtacatttggtggtgtgcgatgcacagagtagaaaccaaataacacacacattt
agaggcatttcactcctcataggcttcatttaccaaaaatatcatgaaaccataactacattaatattacaaaaagcctgtattattctcaaaattcctt
tatctatgtccatagttgtagctaggactgtattccttagcccaccaaaaagaagtttcttgttatccttactaggtaaaaatcagtttgataattgata
ttatgtgatgcagttctgtcattggctgctggttttatattcctctctagaaccaggtagcgataccaacggctttaaacaacatggtagctccagtgtc
tggaaatgatgttggaatccgcagagcagaaatcaagcaggggattcgggaagccatattatgtaaagatcaggatggaaagattggccttcgcctgaaa
tccattgacaatggaatctttgtgcagcttgtacaaaccaactctccagcatccttggctggcctgaag
3' EST: CX967845
[+]ctggaatttaagagaaaccaaatgtaaaccagttactgctcaaacacgaaggctggaattcactgaaacacagcagtagctcagtgcttagggactacag
catgcatgttagccaatcagagtgagcctttccctagagagtctacaaatacaggtgcaggggatggagctaccataataataagaaagtcagcagcaaa
cagtgaggagatccttatctaaaatacattcttgatttcagagacgttaagctaaattacagttagtatcctgatctgtcgatagaaatttattgcatgc
atttgacattcctgccttacattatgaaaatagctatctcatacatgaaatataaaattacagcatatttatacagcaggcaagccttctgagctgcaga
aagacatgcctacaaacagaatagtgcacttgcctcctggtttggatcagatgtataatgggactgagtatggctcatggctgaagcactgtacagaaga
attaactcttgcattcctataccagtgacagacagactttagttctatgtggcaagcagttctgcaacaccaaaagttgcctatcctggatctagagcag
aagtccagatgttgcactacaactcccagcatcccacagacagtggtagtcaacatctggaagttggaccgttgcccagaactgatcacggtacaggtgt
ttgtttgctcctagtaggattttaaagaattagaaatcatcaaaattaggtcattgcttccaacagaaaataattatacatgttctaccccctgctagag
ggacagtagcacacacagtctaggtggctagctatttgcttgctcagttatacaatggaaatgctaacatgca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: