XB-CLONE-3561751
Clone Name: IMAGE:7711149
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Gene:
papss2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967400 [+]
cgcaatcccgtgcataacggacacgcattgctcatgcaggacacccggcgccacctgctgtccaggggttacaagtgccccgttctcttgcttcatcccc
tcgggggttggacaaaggacgacgatgtgcctttggactggcgtatgaagcagcacgatgctgtgctggaggagggggtcctggatcccaagaccaccat
tgtggccatcttcccatccccaatgctctatgccggacccaccgaggttcagtggcactgcagggcccggatgattgcggggtccaacttctacattgtt
ggccgtgacccagcgggaatgcctcacccggagaccaagcaggatctgtatgaagtgacacacgggggcaaggtgctgagcatggcccccggcctgacat
ctgtcgagatcattccattcagagtcgccgcgtacaataaaaagaataaagccatggagttttatgacaaggaaagacacgatgagttcgacttcatctc
cggcactcgaatgaggaaactggcccgggaaggtcagaacccacccgacggcttcatggcccccaaagcctggaaagtcctaactgattattactgctcc
ttggagaagaactgttgactctgaagccgcgccggagagattgtagcctcgttattttctaactaacaattattttatacgccaattagtacacaaatca
atttttatctttgcaatgcttctttcaatggctctttttgtactaaattgaaacgcgccggctagatatggcgttggtgcccataaatctcagtacgtag
atacaatgtgcaatggaatcta
3' EST: CX967399 [+]gaatacagaaatatttattctatatgaaatgtgagcattcgtatcacatctataggggggcacattataaagcataaaggtatcaatatcttaaaaagac
acaagagtcctgtcttgtccataataaatagatttctttacaataaaaaatcagctacaaaaatgattctgatgaaagtgtaatcaaactactatacata
gcaagcagttctgcattatccttcatggtcacccaagagttctgttacagcattaggcagtagcagcgccccctgcaggaatgggaatctctgtgcatgg
ctggctgattaactacggaacgggcaacctcccattaataagggctccatccttactgtcccaggacccccctattccccagtcctgcccctgtggtgga
agttgccaacccctatataagtgtgtggtagcacccatttttggctaaaattccacatttaattaagacatttacaatttaaccccttcagaaaggcaca
gacagaacagtaaagggcaagtaagagcactggggcgcacatttggtccattgttacatatgaggaggtttaaataattcacacaaagataaaaaacata
caaatctcatattaaagcattttcacatattactggggtaaaaacaaaacgaaaaaattgccatgtcctgcggcctaaatattccatgtgatttcccctg
ataataccctgtttgaaaggattaaattacatttttttaaaaaaatatcttctattaccatgtaattcttcccatgtgttctagggattatataattgta
caaatataaatctggggtaagaattaagacgctancataaatatgataaggcatttgcctgtttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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