XB-CLONE-3565851
Clone Name: IMAGE:7709469
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Gene:
lamb2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964621
[+]
tcagaaagcactaggagaagtagaagtgcttttccaaaaggtggctgaagcaaaggtaaaggcagacagagcaaaacagagggcacaagccaccctcgac
agggcaaatgaaacaaaggcaagggtggaacagtccaataaggagctgcgggaactcatccagcaaatcaaggactttctgaaccaggaaggtgcagacc
cagacagcattgagatggtggcaagtcgtgtcttggatctcacaatccctgcaaccccaaagcaaatccagcgtcttgctgaggaaatcaaggacagagt
gaaaacacttgcaaacgtggatgccattctggaccaaaccgctgctgatgtgcgcaaagcagagcaactgctgcatgaagccaaaagagccaagaaccga
gccgagaatgtaaagaacacagccgagtctgtgaaaagggccctggatgatgctaggagagctcagaatgctgctgaaggagctatccaaagcgccaatg
atgacatcaaagacacggagaggaaattaacagcaattcagacagcaacatctagtgcagagaatcagcttaatgatgctatggatcgagtaggaaatct
ggataaacaaattgacggtctgaaaatgaaaagggccaacaacagcttggctgccagccgtgcagaagaatctgccactgcagcactggataaagcaaat
gatgccaagaagatcttagaaggacaactggcagacaaatacaaaactgttcagaatgttgttgatcgaaaagctaagaacatttaagaggcgaagacaa
aagcggaccaacttcaagatgaggcaaagaaactc
3' EST: CX964620
[+]atgaaaatacagtttaaagcaacccatgggagacatatattttttaatataatttcaagatatattacttttttcagtgctttaatctttaatgccagct
aggtcagtgactataaatgtagggatgttagaaaaaagttggccattcccaaacaattatatcactgcaatatagtcataagtgagtctgagtgaaacat
tgagctggttgatgactggtcaggatcaggatattggcagctttttatgcattcccagagacagagatgagacccaagttactctgcaccctagagaaga
ataagtgttacaccccaacccctaaattgaagctggaagcaacattttggccttataatgtgtaatatagctggaaaaaaatggaaaatccctttataag
atgttctccattggtatggtttgaaagttgtaggccttgatgtatgaaatcatagggctgtctctcaccacacttcctttgctatttagaaggggtctga
gaagtgttgacagtcctgcacttctaactggcctactacagcaccagtttttttgttcccatatgtctatattaactttgtagtaacaatgcaacaaaaa
atattttgtttctttaaaaatgtgtttaaagagcattctgttgcattcacattgcccacatatactagtttctgtggcctgcttaacaacagtctaggac
caggcccagactggcaatctgtgggttcaggcaaattccagaggggctgctgcaagatgccatagacagtcactgtttagtgacagtggttcccaaaatg
ttgtctggtccacttggggtgggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: