XB-CLONE-3571931
Clone Name: IMAGE:7702562
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Gene:
tmem243
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC118807 [+]
CAGCTGTCTCTCTCTCCCTCTCTATAAAGAATATGACACTACCGTCTGGGAAGATTTCTATTGATCCAGATGCATAATAAAACAATTAATGGTTATGGAT
GTTACCGAACTTAATATTTTCTACTTTACATACTGAAAGGTAACTAGAAACAGGATAGGTCTGGAATCCAAAAAGGAAAGAACCCAGCCTCCCCAAACAC
CAGTTATTTTACAAAAACATGTTGTAATACAATCTTTTCGACAAACCTGTATCTCTGTATATATTGTGAAATCTTCAATAAAAGTTTTTTAAAATGACAA
TGATTGTATTACACCTTGCATGTAGCCACGCCTTTCTGTCAGGCAGGAAAAGCTTTATTTGTTGACAATTCGATATGTTTTTACAGGAAGACGCTCATCA
TGAACTAAAGATTTTTCTTCCAAGCCCCAGACTGGCACTTCAAGTGTAAATAGCCCATGAGCAAACATTCCATGCCGTGTTCTAAAGGTGCCTACTTTTC
TGCTGTATGGTAGATCCTTACACAGACAGCTAAGGTTAATGACTGAAGAAGCCAGTCCGTTTATCATGGAGGACTATACCACCAGAACCTATGGAACTGG
GGGCCTAGACAACAGACCTTTGTTTGGGGAGACCTCTGCAAGGGACAAAATCATTAACCTAGTTGTTGGTGGACTAACATCTGTGCTTGTACTTGTAACA
ATCGTCAGCGCTTTTGTTTTTCCACATACCCCTCCAAAACCAGTGAATATTTTCTTTGCCATCTGCATTTTCTTGTGCTCAGTAACTGGAATTATTCTAA
TTTTCTGGTACAGACAAGGAGACCTAGAACCAAAATTTAGAAACCTCATCTATTATATTTTGTTTTCAATTGTCATGTTATGCATATGTGCCAACCTATA
TTTTCATGACGTGGGGAAATGAAGCATGACCAATCAGAATTGCACTGGAAACAATGTTCTTTGAAGGCACTACAGGAGGAAGGAGAATGGCTGAACAAAG
GACTTGTGATTCTTTTATGCAAGAAAATGGCTTATACTATAAGACTTTTTTTTTTAAATAGTGTTGCACTTAATCCTCAAGTGGAATTCCCTTTGCCAAC
CTGGACACAAAGAAACACAATCTTGGCACCGCATATGTGAAATATTCTTTATTTTGTTTAAATATAATACAGTGGTTTTTTTCTACTATGCAACAAATGC
CAGTATCTACTACTTCACATTAACAGAACAGTTATGTTAGGTTGTACAGGACAATATAAGGGTTTAATAAATTAAAAAAAAAAGTGAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX938468 [+]gtctctctctccctctctataaagaatatgacactaccgtctgggaagatttctattgatccagatgcataataaaacaattaatggttatggatgttac
cgaacttaatattttctactttacatactgaaaggtaactagaaacaggataggtctggaatccaaaaaggaaagaacccagcctccccaaacaccagtt
attttacaaaaacatgttgtaatacaatcttttcgacaaacctgtatctctgtatatattgtgaaatcttcaataaaagttttttaaaatgacaatgatt
gtattacaccttgcatgtagccacgcctttctgtcaggcaggaaaagctttatttgttgacaattcgatatgtttttacaggaagacgctcatcatgaac
taaagatttttcttccaagccccagactggcacttcaagtgtaaatagcccatgagcaaacattccatgccgtgttctaaaggtgcctacttttctgctg
tatggtagatccttacacagacagctaaggttaatgactgaagaagccagtccgtttatcatggaggactataccaccagaacctatggaactgggggcc
tagacaacagacctttgtttggggagacctctgcaagggacaaaatcattaacctagttgttggtggactaacatctgtgcttgtacttgtaacaatcgt
cagcgcttttgtttttccacatacccctccaaaaccagtg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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