XB-CLONE-3630070
Clone Name: IMAGE:7666061
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Gene:
cdk5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC118780 [+]
GAACAGTGAGGGCGGCCTGCCGGTGCTATCATGCAGAAGTACGAGAAGCTGGAGAAGATCGGGGAAGGTACCTATGGGACAGTGTTCAAGGCCAAGAACC
GGGAGACCCATGAGATTGTGGCGCTGAAACGAGTGCGCCTTGATGACGATGATGAGGGGGTGCCAAGCTCAGCGCTGCGGGAGATCTGCCTCCTCAAGGA
ACTCAAGCACAAGAACATCGTCAGGTTACATGATGTGCTGCACAGTGACAAGAAGCTGACGCTGGTGTTTGAGTACTGTGACCAGGATCTGAAGAAGTAT
TTCGACAGCTGCAATGGGGACCTGGACCCAGAGATAGTGAAGTCGTTCATGTACCAACTGCTGAAGGGCCTGGGGTTCTGCCACAGCCGGAATGTTCTGC
ACCGGGACCTGAAGCCACAGAACCTGCTCATCAACAGGAATGGGGAGCTGAAGCTGGCAGACTTCGGATTGGCTCGTGCCTTTGGGATCCCGGTGCGATG
TTACTCGGCTGAGGTGGTTACCCTCTGGTACCGGCCCCCCGACGTGCTCTTTGGGGCTAAACTCTACTCCACCTCCATAGATATGTGGTCGGCCGGCTGC
ATATTTGCAGAGTTGGCCAATGCCGGCCGCCCACTGTTTCCCGGAAATGATGTCGACGACCAACTGAAGAGGATCTTCCGGTTGCTGGGGACCCCCACGG
AGGAGCAGTGGCCAGCCATGACCAAACTTCCCGACTATAAGCCCTACCCCATGTACCCCGCCACCATGTCTCTAGTGAACGTTGTGCCCAAGCTGAATGC
CACGGGGCGGGACTTGCTGCAGAATCTGCTGAAGTGCAACCCGGTGCAGAGAATCTGTGCGGATGAGGCCCTCCAGCACCCTTACTTTGCTGACTTTTGC
CCCCCGTAACCTGCCCTTCCCTCTGAGCCCTGAAGATCATGTCCCCCCCTGCCCTTCTGTGTCTCTGATGTCACCAACTGTCACACCTCCCCCAGCGACC
CGCCAGTTACTGGCACTGACTCCGCCCATGGCCAGCGCAGCCGGGGAAGAATCAACATTCTGATTGGCTGCCAGTCTGTGGGGCCCTGCGCTGTCATTGG
TTCCTGATCCTGCACTGGCCGGAGGATCATGTGACTGGCCATGCCCACTACTGGAATAAAGTGATGGATTCGGGGTGCAACCAGTTACCCCAGAACATTT
AACTGCTTAAGTGCCAGAGGCCTAACGCTACGGCTGCCATTGGTTCAGGCTCAGCCCTTCCCATCCGGATGCCCCTCCCCGTAATGTTCTCCCCCCAGAC
ATCAGAGGGCTCCTCCCCCTTATTATTTATTTGGGAGGCATGAACTCCTCCCCCCCAGGTATCAGGTGTATATTTTAATATTAATTAATTATGTTGAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX851832 [+]acagtgagggcggcctgccggtgctatcatgcagaagtacgagaagctggagaagatcggggaaggtacctatgggacagtgttcaaggccaagaaccgg
gagacccatgagattgtggcgctgaaacgagtgcgccttgatgacgatgatgagggggtgccaagctcagcgctgcgggagatctgcctcctcaaggaac
tcaagcacaagaacatcgtcaggttacatgatgtgctgcacagtgacaagaagctgacgctggtgtttgagtactgtgaccaggatctgaagaagtattt
cgacagctgcaatggggacctggacccagagatagtgaagtcgttcatgtaccaactgctgaagggcctggggttctgccacagccggaatgttctgcac
cgggacctgaagccacagaacctgctcatcaacaggaatggggagctgaagctggcagacttcggattggctcgtgcctttgggatcccggtgcgatgtt
actcggctgaggtggttaccctctggtaccggccccccgacgtgctctttggggctaaactctactccacctccatangatatgtggtcggccggctgca
tatttgcagagttggccaatgccggccgcccactgtttcccgganatgatgtcgacgaccaactgaagaggatcttccggttgctggggacccccacgga
ggagcagtggccagccatgaccaaacttcccgactataagccctaccccatgtaccccgccaccatgtctctagtgaacgttgtgcccaagctgaatgcc
acggggcgggacttgctgcagaatctgctgaagtgcaacccg
3' EST: CX851831 [+]caacataattaattaatattaaaatatacccctgatacctgggggggaggagttcatgcctcccaaataaataataagggggaggacccctctgatgtct
ggggggaaaacattacggggaggggcatccggatggaaagggctgaccctgaaccaatggcagccgtagcgttaggcctctggcacttaagcagttaaat
gttctggggtaactggttgccccccaaatccatcactttattccagtagtgggcatggccagtcacatgatcctccggccagtgcaggatcaggaaccaa
tgacagcgcagggccccacaaactggcagccaatcaaaatgttgattcttccccggctgccctggccatgggcggagtcagtgccagtaactggcgggtc
cctgggggaggtgtgacatttggtgacatcaaaaacacaaaagggcagggggggacatgatcttcagggctcaaaggaaagggcaggttacggggggcaa
aagtcagcaaagtaagggtgctggagggcctcatccgcacaaattctctgcaccgggttgcacttcagcaaattctgcagcaagtcccgccccgtggcat
tcagcttgggcacaacgttcactaaagacatggtggcggggtacatggggtagggcttatattc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: