XB-CLONE-3916867
Clone Name: IMAGE:7091960
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Gene:
drg2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654564 [+]
taattatccccccccccccccggcagagatggggatcctggaaaagatttcggagatcgaaagggagatcgccaggacccagaagaataaggccactgaa
taccatctgggcctgctaaaggccaaacttgcaaagtaccgagctcagctcctggaaccatccaaatctgcagccaacaaaggagagggctttgatgtta
tgaaatctggagatgcgggggtggctctgattggctttccaagggggggcaagtctaccttcttgagcttgatgacatccacagggagggaagcggaggc
ctacnantttactacactcacttgcatcccangagtgatcgagtacaagggggccaatattcaacttcttgatctcccaggaattattgaaggagcagct
caggggaaaggaagaggtcggcaggtcattgcagtggcacggacatcagacgtggttataatgatgttggatgccacaaaaggagaggttcaaagatcat
tactggagaaagagttggaatcagtgggcattcgtctaaacaaagacaaacctaacatatacttcaagccgaagaaaagaggcggaatctcatttaactc
cacagtacccctaacccaatgttcggagaagctggtgcagcttatctgcatgatacaaaatgttaatgcagaagg
3' EST: CN319094 [+]ttttttttttttttcattgttaggaaggctccaagtctgaatccatttatttagtgctgttcagtaaatcctatggaagtccatggaaaagaatattccc
cccagtcactttttaataattggtggctattttcacgatttttttcacactactaaattcttcccacagtcttcaagagctaccattgaccttaaccctt
tctgcaaagagatggcaagaaactgggggaaatggcttcttctctgaagatgaaagcatgtgatatgcttaaagcaatttatttcttgacgacctggata
acatcttcatgttccatattgtgggtcaggcctactctctggggactgtatttggtgctggtgccccaaaccagggcatatttaaactggctggcaagtg
ttctgtgtattcgatgacacacatgctccactgaagctcctttccgtaggatgattgcatcagcaaaatctggtctctctcctcttttctttgtgtaaat
acaggtaagtgcaaggtattcccagagcatctccagtaaataatccaaatttagcttcatcccacagctgattaccacactattaggctgtctggctagt
cggtctacctcctccatggaaatctgatcaattttgttatatacatagagacatggcatgtaaactctgtttcaacgataacatctatgaaatcttcagg
cctgcaatcctctcggaacagccacctctgcattaaacattttggattcctgcaaaataagctggcacagcttctcccaaacccggggttaggggaaacg
gggggagtaaatgaaaattcccccctcctttccttcggctttgaagttaattgtttggggtttggttttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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