XB-CLONE-4351943
Clone Name: IMAGE:7027213
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Gene:
ppp1r14a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080468 [+]
CGACAGGGATTGTCGGTGTTAGCGCTTGGGGTTTGAGGACTCTCCCCAGCTGATCCTGCTATCCCCACAACTGTTAAAGGACGCTCTTCAGTGCAGTTAA
TCTCCGCCGAGCGCTGGAGCGGTGCTACAGTTTGCATGGTATTTGGTGCTCCCCTGAGTGGCTTGTATTGATTGGCTGAGCAGGCTGAGGCGCTTACCCG
TGACTATCGCAGCCTGTGACTACCTCGCTAGTGAAGGGTCTCCGTCTCACTGTCCGGTAGTACCAGCAATGGCAGCCAATAAGGTCGGCAAAAGATTCCC
AAATAAGCCTCATTCCCCATCCAAGACTAGAGACTCCAATCAAGGCATCCAGAGGCGACAGGCGCGGGTTACTGTCAAGTATGATCGCAAAGAGTTGCAG
AAGCGGCTGGATGTGGAGAAGTGGATAGACGAGCAGATGGAGGAGCTGTACCTAGGGAGGGAAGTAGATATGCCAGATGAGGTCAACATTGATGATCTGT
TGGACTTGGAGACGGATGAAGACAGAAGACAGAGCTTGCAGGTCATCTTGAAGTCTTGCACTAACAACACAGAGGCGTTCATTCGGGAGCTTCTCTTAAG
GCTGAAGGGACTGCAGAAGCAAACTCTAATTAAAAAGAATGGACTGGAGGTTTCCAGTGAGGAGCACATTGTGGAACATGCGTAACCCACCATATCCCTC
AAAGCTCTGTAAGCGGTTTATTACCAGAGAACAAGGACCGTCTACAACGCTAAGGAAAGGAATGAATGAAGAGTTGCAGAGACTTCAGACACTAAAGATG
GACAATTTTTGATGTCTTTCTTTCTGATGACATTTCTAACATGAGAATTTACAAGACAATTTTGTGTTTCAATAGAAGTTTTTTGTGTGCCCAATTATTA
TAACTCTTTTCACAGATTGTGTTGCCCACATTCATCTTTATTTGAATGCCTAACACTCATGAATAGAGTTCCTAAACATACTATTAATTACAATTCACCC
CTGTTTTGCCTGTTGTAAAGTTATGTTCAGTACTATATTTTTAAATCAGAACTTGGATTTTACTTTGGAGGAAGATCAGCTGGTGTGGTGAGACCCAACC
CTGTCTGGGCATTGTGGATGGCCCCTGTATTGCCTATGTGAGGGGAGTCATGTCCTGTATTGCAAAGTATAGGGAATCCTGTAATGTACATAGACAACAC
CAGACCCTGCCTTTTTAAAGTCTATGTGGAGCCTGTACCTTGTATGATGTACAGTTTGGGGGGAAATTAGGCCCTTCGTTTGGCTGTTTCTGGAGAAGCC
ATGATGAACCAAACCAGGAGAAATACAAGTAGGTGCCCTTATGCAAGAAAAAGCTGGTTTATTTACTCATTTCTTACATTGTGTTTAGTTTGACACTTAA
AGCCAGGTAGCTGGTCACAAATCACGTGAAATGCTATAGAGTTGTCTCCCATCTGCTCAGCACTCAGCACACTTTTTTTTTTCATTTCTTTGAATTAGTA
TTAGCTATACACAGAATCCATAAATTGGGCATTCATCAGAATACCAGTTCCTTATCCAAATATTTGACCAAATACCTACACACATTTTCATGTTTGAGGA
AAAAAAACAGAAAATGCATTAATTGAAAAAGTGTCAGTTCCTTAGTGTTTTAAAGTCAAATGATTTTCTGGTTTGCATTGAGTCCGCTAAACTCTGATTT
GTTCGAATCCTGGTTAAAAAAAAAAGTGCTGCAATTTGTCTAAATTCTGAACTTGAACTGAATAGTGGATTCAGTGTGTTCCTAATTAGAAAATATACTA
ATCTTTGACTATTAACTAAAGTGGCATAAAGGCACAGACACACATACACATATATATTTACAAAAATTGGGTCAGTTTGTAATACACAAATCAAGCAGCT
GGCTTACTCACACTGTAATAGCTTTAAATACCGTTATTCATATCCATACACTGTTATATATTAAATATGCATATTGTATATATTTATATGGACAGGCCAT
GTAGCTTCTGTAATGCTCTATACACTACAGGTTGCGTTGGATACAACGGCCATGCCACTTAAGGAAGGTCACTTTATAGTTTAACAAGTTAGTCTTATGT
TTTGGTTTTAATAAATTGTTTCTCTTTCAAAAAACAATAAAAAAAAAAAAAAAA
5' EST: CF784570 [+]taattaatccccccccccccccgacagggattgtcggtgttagcgcttggggtttgaggactctccccagctgatcctgctatccccacaactgttaaag
gacgctcttcagtgcagttaatctccgccgagcgctggagcggtgctacagtttgcatggtatttggtgctcccctgagtggcttgtattgattggctga
gcaggctgaggcgcttacccgtgactatcgcagcctgtgactacctcgctagtgaagggtctccgtctcactgtccggtagtaccagcaatggcagccaa
taaggtcggcaaaagattcccaaataagcctcattccccatccaagactagagactccaatcaaggcatccagaggcgacaggcgcgggttactgtcaag
tatgatcgcaaagagttgcagaagcggctggatgtggagaagtggatagacgagcagatggaggagctgtacctagggagggaagtagatatgccagatg
aggtcaacattgatgatctgttggacttggagacggatgaagacagaagacagagcttgcaggtcatcttgaagtcttgcactaacaacacagaggcgtt
cattcgggagcttctcttaaggctgaaaggactgcagaagcaaactctaattaaaaagaatggactggaggtttccagtgaggagcacatttgtggacat
gcgtaacccaccatatccctcaaagctctgtaagcggttttattacagagaaacaaggacccgtctaccaacgcttaaggaaaaaggaatgaaatgtaan
aagtttgcaaaagaactt
3' EST: CF784569 [+]tttttttttttttttattgttttttgaaagagaaacaatttattaaaaccaaaacataagactaacttgttaaactataaagtgaccttccttaagtggc
atggccgttgtatccaacgcaacctgtagtgtatagagcattacagaagctacatggcctgtccatataaatatatacaatatgcatatttaatatataa
cagtgtatggatatgaataacggtatttaaagctattacagtgtgagtaagccagctgcttgatttgtgtattacaaactgacccaatttttgtaaatat
atatgtgtatgtgtgtctgtgcctttatgccactttagttaatagtcaaagattagtatattttctaattaggaacacactgaatccactattcagttca
agttcagaatttagacaaattgcagcacttttttttttaaccaggattcgaacaaatcagagtttagcggactcaatgcaaaccagaaaatcatttgact
ttaaaacactaaggaactgacactttttcaattaatgcattttctgttttttttcctcaaacatgaaaatgtgtgtaggtatttggtcaaatatttggat
aaggaactggtattctgatgaatgcccaatttatggattctgtgtatagctaatactaattcaaagaaatgaaaaaaaaaagtgtgctgagtgctgagca
aatgggagacaactctatagcatttcacgtgatttgtgaccagctacctggctttaagtgtcaaactaacacaatgtaaagaaatgagtaaataaaccaa
gctttttcttgcataagggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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