Return to search results Title: Induction of dorsal mesodermal genes by pinhead and chordin

Summary

Xenopus embryonic ectodermal cells are responsive to various inducing factors. Mesoderm is specified and patterned by extracellular factors including FGF, Nodal, BMP and Wnt families. Pinhead is another secreted protein implicated in mesoderm formation. We found that Pinhead physically interacts with and antagonizes ADMP (anti-dorsalizing morphogenetic protein) that acts as BMP-like protein to promote ventral mesoderm formation. ADMP and BMPs have been shown to cooperate to activate phospho-Smad1 signaling to lead to ventral mesoderm development. Since Chordin is a BMP antagonist, we hypothesized that Pinhead together with Chordin can promote downregulation of phospho-Smad1 signaling that leads to dorsal mesoderm development. RNA sequencing revealed that Pinhead and Chordin synergize in dorsal mesoderm formation in ectodermal explants.

Contributors: Keiji Itoh, Olga Ossipova, Sergei Sokol

Experiment Type: Eight samples are collected for RNAseq from four groups with duplicates. One group is control ectodermal explants (Co), the second is Flag-Pnhd expressing explants (Fpinh), the third is Chordin expressing explants (Chor) and the fourth is Pinhd and chordin expressing explants (Chor-Pinh).

Experiment Reagents: chrd.L, pnhd.L, Tg(Xla.pnhd.L-FLAG).

Article: XB-ART-58208, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
animal cap - animal cap explant - NF15 GSM5137445  GSM5137446  RNA-Seq [-] [+] Background: Xla.pigmentedNasco Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area explant   NF stage 9 animal cap Conditions: Tissue Assay Stages ChIP Antibody animal cap NF stage 15      animal cap - chrd + animal cap explant - NF15 DEG GSM5137447  GSM5137448  RNA-Seq [-] [+] Background: Xla.pigmentedNasco Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection chrd.L chrd.L NF stage 2 (2-cell) animal hemisphere explant   NF stage 9 animal cap Conditions: Tissue Assay Stages ChIP Antibody animal cap NF stage 15      animal cap - chrd + pnhd + animal cap explant - NF15 DEG GSM5137451  GSM5137452  RNA-Seq [-] [+] Background: Xla.pigmentedNasco Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection chrd.L chrd.L NF stage 2- animal hemisphere mRNA injection pnhd.L pnhd.L NF stage 2 (2-cell) animal hemisphere explant   NF stage 9 animal cap Conditions: Tissue Assay Stages ChIP Antibody animal cap NF stage 15      animal cap - Xla.pnhd-FLAG + animal cap explant - NF15 DEG GSM5137449  GSM5137450  RNA-Seq [-] [+] Background: Xla.pigmentedNasco Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection Tg(Xla.pnhd.L-FLAG) pnhd.L NF stage 2 (2-cell) animal hemisphere explant   NF stage 9 animal cap Conditions: Tissue Assay Stages ChIP Antibody animal cap NF stage 15

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